Abstract
Ascorbate peroxidase(APX) is a class I peroxidase that catalyzed the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. This enzyme has a key function in scavenging reactive oxygen species (ROS) and the protection against toxic effects of ROS in plants. Here we reported the identification of an APX gene from a cDNA library of Grimmia pilifera. The full-length of the gene was cloned by 3′-RACE approach, and named as GpAPX. The predicted proteins of GpAPX consisted of 256 amino acid residues with a calculated molecular mass of 28.2 kDa, and displayed high similarity to other plant proteins. It also contains three conserved domains and two signatures. QRT-PCR analysis suggested that the expression of GpAPX gene was induced in both dehydration and rehydration. Its expression level increased under drought stress, inhibited at the begining of re-watering, and peaked on 12h after re-watering. The results demonstrated that the expression of GpAPX gene closely related to dehydration and rehydration, and its isolation established a good foundation for further study on the resistance of Grimmia pilifera to drought stress.
This work is supported by The National Natural Science Foundation of China (31070180), Overseas scholars cooperation project of Heilongjiang Education Department(1151HZ022), The Agriculture research project of Heilongjiang Science and Technology Department (GC06J101) .
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Badawi, G.H., Yamauchi, Y., Shimada, E., Sasaki, G.R., Kawano, N., Tanaka, K., Tanaka, K.: Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts. Plant Science 166, 919–928 (2004)
Bowler, C., Van, M.M., Inze, D.: Superoxide dismutase and stress tolerance. Annual Review of Plant Biology 43, 83–116 (1992)
Guo, Z., Ou, W., Lu, S., Zhong, Q.: Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry 44, 828–836 (2006)
Asada, K.: Ascorbate-peroxidase;scavenging system of hydrogen peroxide in chloroplasts. Tanpakushitsu Kakusan Koso 33, 2957–2964 (1988)
Teixeira, F.K., Menezes-Benavente, L., Galva, V.C., Margis, R., Margis-Pinheiro, M.: Rice ascorbate peroxidase gene family encodes functionally diverse isoforms localized in different subcellular compartments. Planta 224, 300–314 (2006)
Sharma, P., Dubey, R.S.: Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation 46, 209–211 (2005)
Yoshimura, K., Yabuta, Y., Ishikawa, T., Shigeoka, S.: Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiology 123, 223–234 (2000)
Sgherri, C.L.M., Maffei, M., Navari-lzzo, F.: Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. Journal of Plant Physiology 157, 273–279 (2000)
Shi, W.M., Muramoto, Y., Ueda, A., Takabe, T.: Cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana. Gene. 273, 23–27 (2001)
Ma, L.B., Zhang, F.Y., Ma, C.Y., Wang, S.J.: cDNA cloning and sequence characterization of an ascorbate eroxidase from marine red alga Porphyra yezoensis. Journal of Fishery Sciences of China 12, 281–287 (2005)
Lee, Y.P., Kim, S.H., Bang, J.W., Lee, H.S., Kwak, S.S., Kwon, S.Y.: Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. Plant Cell Reports 26, 591–598 (2007)
Lu, Z.Q., Liu, D.L., Liu, S.K.: Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis. Plant Cell Reports 26, 1909–1917 (2007)
Yan, J., Wang, J., Tissue, D., Holaday, A.S., Allen, R.: Photosynthesis and seed production under water-deficit conditions in transgenic tobacco plants that overexpression an Arabidopsis ascorbate peroxidase gene. Crop Science 43, 1477–1483 (2003)
Oliver, M.J., Bewley, J.D.: Desiccation tolerance of plant tissues: A mechanistic overview. Horticulture Reviews 18, 171–214 (1997)
Narendra, S., Venkataramani, S., Shen, G.X., Wang, J., Pasapula, V., Lin, Y., Kornyeyev, D., Holaday, A.S., Zhang, H.: The Arabidopsis ascorbate peroxidase 3 is a peroxisomal membrane-bound antioxidant enzyme and is dispensable for Arabidopsis growth and development. Journal of Experimental Botany 57, 3033–3042 (2006)
Henrissat, B., Saloheimo, M., Lavaitte, S., Knowles, J.K.: Structural homology among the peroxidase enzyme family revealed by hydrophobic cluster analysis. Proteins 8, 215–217 (1990)
Park, S.Y., Ryu, S.H., Jang, I.C., Kwon, S.Y., Kim, J.G., Kwak, S.S.: Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweet potato and its expression in response to stress. Molecular Genetics and Genomics 271, 339–346 (2004)
Oliver, M.J.: Desiccation tolerance in vegetative plant cells. Physiologia Plantarum 97, 779–787 (1996)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer-Verlag GmbH Berlin Heidelberg
About this chapter
Cite this chapter
Song, X.H., Sha, W., Jin, Z.M., Zhang, Y.F. (2012). Cloning, Characterization and Expression Analysis of a Cytosolic Ascorbate Peroxidase Gene from Grimmia Pilifera . In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_53
Download citation
DOI: https://doi.org/10.1007/978-3-642-27537-1_53
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-27536-4
Online ISBN: 978-3-642-27537-1
eBook Packages: EngineeringEngineering (R0)