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Structural and Functional Features of Peroxidases with a Potential as Industrial Biocatalysts

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Biocatalysis Based on Heme Peroxidases

Abstract

This chapter begins with a description of the main structural features of heme peroxidases representative of the two large superfamilies of plant–fungal–bacterial and animal peroxidases, and the four additional (super)families described to date. Then, we focus on several fungal peroxidases of high biotechnological potential as industrial biocatalysts. These include (1) ligninolytic peroxidases from white-rot basidiomycetes being able to oxidize high redox-potential substrates at an exposed protein radical; (2) heme-thiolate peroxidases that are structural hybrids of typical peroxidases and cytochrome P450 enzymes and, after their discovery in sooty molds, are being described in basidiomycetes with even more interesting catalytic properties, such as selective aromatic oxygenation; and (3) the so-called dye-decolorizing peroxidases that are still to be thoroughly investigated but have been identified in different basidiomycete genomes. The structural–functional description of these peroxidases includes an analysis of the heme environment and a description of their substrate oxidation sites, with the purpose of understanding their interesting catalytic properties and biotechnological potential.

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Notes

  1. 1.

    Concerning PeroxiBase, note that (1) the database although under permanent updating is not exhaustive; (2) hypothetical peroxidase genes from genomes, and partial sequences, are included; and (3) the information cited here corresponds to January 2010.

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Acknowledgments

The authors thank Martin Hofrichter (University of Zittau, Germany) for useful information on basidiomycete heme-thiolate and DyP-type peroxidases. The financial support of the BIORENEW EU-contract on “White Biotechnology for added value products from renewable plant polymers: Design of tailor-made biocatalyst and new industrial bioprocesses” (NMP2-CT-2006-026456) and the RAPERO Spanish Biotechnology project on “Radical peroxidases” (BIO2008-01533) is acknowledged. F.J.R.-D. thanks the Spanish MICINN for a “Ramón y Cajal” contract.

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  1. Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040, Madrid, Spain

    Francisco J. Ruiz-Dueñas & Angel T. Martínez

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  1. Francisco J. Ruiz-Dueñas
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  1. , Centro de Química, Benemérita Universidad Autónoma de Puebl, Ciudad Universitaria, Puebla, 72570, Mexico

    Eduardo Torres

  2. , Biotechnology Institute, Autonomous University of Mexico, Av. Universidad, Col. Chamilpa 2001, Cuernavaca, Morelos, 62210, Mexico

    Marcela Ayala

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Ruiz-Dueñas, F.J., Martínez, A.T. (2010). Structural and Functional Features of Peroxidases with a Potential as Industrial Biocatalysts. In: Torres, E., Ayala, M. (eds) Biocatalysis Based on Heme Peroxidases. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12627-7_3

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