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Cultivation of Anaerobic Microorganisms with Hydrocarbons as Growth Substrates

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Handbook of Hydrocarbon and Lipid Microbiology

Abstract:

Microorganisms utilizing hydrocarbons anaerobically as organic growth substrates are grown in defined anoxic media with sulfate, nitrate or other electron acceptors. Cultivation of such microorganisms is technically often more elaborated than that of other anaerobes. Special technical measures include, for instance, dilution of potentially toxic hydrocarbons in an inert carrier phase, and precautions to aviod diffusion of traces of oxygen through stoppers during long incubation times. Furthermore, contact of liquid hydrocarbons with stoppers should be avoided.

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Widdel, F. (2010). Cultivation of Anaerobic Microorganisms with Hydrocarbons as Growth Substrates. In: Timmis, K.N. (eds) Handbook of Hydrocarbon and Lipid Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77587-4_298

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