Skip to main content
SpringerLink
Log in

Production of Alkaloids in Plant Cell and Tissue Cultures

  • Chapter
Bioactive Molecules and Medicinal Plants

Abstract

A low or no productivity of alkaloids in plant cell cultures can be explained by an insufficient level of cell differentiation. The first strategy described in this chapter for improving isoquinoline alkaloid accumulation is organogenesis and somatic embryogenesis induced by the addition of exogenous growth regulators in Papaver somniferum and Leucojum aestivum cell cultures. The second strategy described is the transformation of medicinal plants (Atropa belladonna, Papaver somniferum, and Leucojum aestivum) using Agrobacterium rhizogenes to form hairy root cultures, which carry with them with the benefits of fast growth and rates of alkaloid production equal to or greater than that found for the intact plant.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Harborne JB (1999) Classes and functions of secondary products from plants. In: Walton NJ, Brown DE (eds) Chemicals from Plants, Perspectives on Secondary Plant Products. Imperial College Press, London, pp 1–25

    Chapter  Google Scholar 

  2. Verpoorte R, van der Heijden R, ten Hoopen HJG, Memelink J (1999) Biotechnol Lett 21:467–479

    Article  CAS  Google Scholar 

  3. Payne GF, Bringi V, Prince C, Shuler ML (1991) The quest for commercial production of chemicals from plant cell culture. In: Payne GF, Bringi V, Prince C, Shuler ML (eds) Plant Cell and Tissue Culture in Liquid Systems. Hanser, Munich, pp 1–10

    Google Scholar 

  4. Fujita Y, Tabata M (1987) Secondary metabolites from plant cells-pharmaceutical applications and progress in commercial production. In: Green CE, Somers DA, Hacket WP, Biesboer DD (eds) Plant Tissue and Cell Culture. Alan R. Liss, New York, USA, pp 169–185

    Google Scholar 

  5. Tabata H (2004) Adv Biochem Eng Biotechnol 87:1–23

    CAS  Google Scholar 

  6. Dräger B (2007) Biotechnology of solanaceae alkaloids: a model or an industrial perspective? In: Kayser O, Quax W (eds) Medicinal Plant Biotechnology, From Basic Research to Industrial Applications, Vol 1. Wiley-VCH, pp 237–265

    Google Scholar 

  7. Schuchmann R, Wellmann E (1983) Plant Cell Rep 2:88–91

    Article  CAS  Google Scholar 

  8. Yoshikawa T, Furuya T (1985) Planta Med 2:110–113

    Article  Google Scholar 

  9. Yoshimatsu K, Shimomura K (1992) Plant Cell Rep 11:132–136

    Article  CAS  Google Scholar 

  10. Laurain-Mattar D, Gillet-Manceau F, Buchon L, Nabha S, Fliniaux MA, Jacquin-Dubreuil A (1999) Planta Med 65:167–170

    Article  CAS  Google Scholar 

  11. Gabrielsen B, Monath TP, Huggins JW, Kefauver DF, Pettit GR, Groszek G, Hollingshead M, Kirsi JJ, Shannon WM, Schubert EM, Dare J, Ugarkar B, Ussery MA Phelan MJ (1992) J Nat Prod 55:1569–1581

    Article  CAS  Google Scholar 

  12. Weniger B, Italiano L, Beck JP, Bastida J, Bergonon S, Codina C, Lobstein A, Anton R (1995) Planta Med 61:77–79

    Article  CAS  Google Scholar 

  13. Guillou C, Beunard J.L, Gras E, Thal C (2001) Angew Chem Int Edit 40:4745–4746

    Article  Google Scholar 

  14. Marco-Contelles J, do Carmo Carreiras M, Rodriguez C, Villarroya M, Garcia AG (2006) Chem Rev 106:116–133

    Article  CAS  Google Scholar 

  15. Sellés M, Bergonon S, Viladomat F, Bastida J, Codina C (1999) Plant Cell Rep 18:646–651

    Article  Google Scholar 

  16. Ilahi I (1983) Pakistan J Bot 15:13–18

    CAS  Google Scholar 

  17. Galewsky S, Nessler C.L (1986) Plant Sci 45:215–222

    Article  CAS  Google Scholar 

  18. Furuya T, Ikuta A, Syono K (1972) Phytochemistry 11:3041–3044

    Article  CAS  Google Scholar 

  19. Ranganathan B, Mascarenhas AF, Sayagaver BM, Jagannathan V (1963) Growth of Papaver somniferum in vitro. In: Maheshwari P (ed) Plant Tissue and Organ Culture. International Society of Plant Morphology, University of Delhi, India, pp 108–110

    Google Scholar 

  20. Carew DP, Staba EJ (1965) Lloydia 35:1–26

    Google Scholar 

  21. Staba EJ (1969) Recent Adv Phytochem 2:75–106

    CAS  Google Scholar 

  22. Ikuta A, Syono K, Furuya T (1974) Phytochemistry 13:2175–2179

    Article  CAS  Google Scholar 

  23. Morris P, Fowler MS (1980) Planta Med 39:284–285

    Google Scholar 

  24. Staba EJ, Zito S, Amin M (1982) J Nat Prod 45:256–262

    Article  CAS  Google Scholar 

  25. Kamo KK, Kimoto W, Hsu AF, Mahlberg PG, Bills DD (1982) Phytochemistry 21:219–222

    Article  CAS  Google Scholar 

  26. Facchini PJ, Bird DA (1998) In Vitro Cell Dev Biol Plant 34:69–79

    Article  CAS  Google Scholar 

  27. Diop MF, Ptak A, Chrétien F, Henry M, Chapleur Y, Laurain-Mattar D (2006) Nat Prod Commun 1:475–479

    CAS  Google Scholar 

  28. Diop MF, Hehn A, Ptak A, Chrétien F, Doerper S, Gontier E, Bourgaud F, Henry M, Chapleur Y, Laurain-Mattar D (2007) Phytochem Rev 6:137–141

    Article  CAS  Google Scholar 

  29. Piñol MT, Palazón J, Cusido R, Serrano M (1996) Bot Acta 109:133–138

    Google Scholar 

  30. Palazón J, Cusido RM, Gonzalio J, Bonfill M, Morales C. Piñol T (1998) J Plant Physiol 153:712–718

    Article  Google Scholar 

  31. Bonhomme V, Laurain-Mattar D, Lacoux J, Fliniaux MA, Jacquin A (2000) J Biotechnol 81:151–158

    Article  CAS  Google Scholar 

  32. Bonhomme V, Laurain-Mattar D, Fliniaux MA (2000) J Nat Prod 63:1249–1252

    Article  CAS  Google Scholar 

  33. Le Flem-Bonhomme V, Laurain-Mattar D, Fliniaux MA (2004) Planta 218:890–893

    Article  Google Scholar 

  34. Endo T, Yamada Y (1985) Phytochemistry 24:1233–1236

    Article  CAS  Google Scholar 

  35. Jouhikainen J, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey K-M (1999) Planta 208:545–551

    Article  CAS  Google Scholar 

  36. Payne J, Hamill JD, Robins RJ, Rhodes M-JC (1987) Planta Med 53:474–478

    Article  CAS  Google Scholar 

  37. Deno H, Yamagata H, Emoto T, Yoshioka T, Yamada Y, Fujita Y (1987) J Plant Physiol 131:315–324

    Article  CAS  Google Scholar 

  38. Sevon N, Hiltunen R, Oksman-Caldentey KM (1998) Planta Med 64:37–41

    Article  CAS  Google Scholar 

  39. Aoki T, Matsumoto H, Asako Y, Matsunagra Y, Shimomura K (1997) Plant Cell Rep 16:282–286

    CAS  Google Scholar 

  40. Giulietti AM, Parr AJ, Rhodes M-JC (1993) Planta Med 59:428–431

    Article  CAS  Google Scholar 

  41. Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Plant Cell Rep 5:239–242

    Article  CAS  Google Scholar 

  42. Hashimoto T, Yun DJ, Yamada Y (1993) Phytochemistry 32:713–718

    Article  CAS  Google Scholar 

  43. Sharp JM, Doran PM (1990) J Biotechnol 16:171–186

    Article  CAS  Google Scholar 

  44. Jung G, Tepfer D (1987) Plant Sci 50:145–151

    Article  CAS  Google Scholar 

  45. Christen P, Roberts MF, Phillipson JD, Evans WC (1989) Plant Cell Rep 8:75–77

    Article  CAS  Google Scholar 

  46. Hartmann T, Witte L, Oprach F, Toppel G (1986) Planta Med 52:390–395

    Article  Google Scholar 

  47. Rothe G, Garske U, Dräger B (2001) Plant Sci 160:1043–1053

    Article  CAS  Google Scholar 

  48. Toivonen L (1993) Biotechnol Progress 9:12–20

    Article  CAS  Google Scholar 

  49. Sevon N, Oksman-Caldentey KM (2002) Planta Med 68:859–868

    Article  CAS  Google Scholar 

  50. Cline SD, Coscia CJ (1988) Plant Physiol 86:161–165

    Article  CAS  Google Scholar 

  51. Eilert U, Kurz WGW, Constabel F (1985) J Plant Physiol 119:65–76

    Article  CAS  Google Scholar 

  52. Millgate AG, Pogson BJ, Wilson IW, Kutchan TM, Zenk MH, Gerlach WL, Fist AJ, Larkin PJ (2004) Nature 431:413–414

    Article  CAS  Google Scholar 

  53. Page J. (2005) Trends Biotechnol 23:331–333

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Groupe S.U.C.R.E.S., U.M.R. 7565 C.N.R.S., Nancy-Université, 54506, Nancy-Vandoeuvre, France

    Dominique Laurain-Mattar

Authors
  1. Dominique Laurain-Mattar
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratory of Biomolecular Technology, Department of Botany, M.L. Sukhadia University, 313001, Udaipur, India

    K.G. Ramawat

  2. Groupe d‘Etude des Substances Végétales à Activité Biologique Laboratoire de Sciences Végétales, Mycologie et Biotechnologie,, UFR Pharmacie Universite Victor Segalen, 146 Rue Leo-Saignat, 33076, Bordeaux cedex, France

    J.M. Merillon

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Laurain-Mattar, D. (2008). Production of Alkaloids in Plant Cell and Tissue Cultures. In: Ramawat, K., Merillon, J. (eds) Bioactive Molecules and Medicinal Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74603-4_8

Download citation

Publish with us

Policies and ethics

Search

Navigation