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Gene Amplification in Dipteran Chromosomes

  • Chapter
Structure and Function of Eukaryotic Chromosomes

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 14))

Abstract

The giant polytene chromosomes characteristic of many Dipteran cells allow fundamental nuclear processes occurring during development to be directly visualized. Polytene chromosomes afford a detailed picture of “large-scale” structures within developmentally regulated interphase cells — features extending over more than about 5–10 kb of chromosomal DNA. Structures, such as chromomeres, constrictions, and puffs, must be disrupted for biochemical studies and cannot currently be reconstituted. However, following Wolfgang Beermann’s recognition that polytene chromosome puffs were manifestations of developmentally regulated gene expression, much progress has been made in understanding the molecular basis of gene transcription. Genetic studies have identified small regulatory regions located near tissue-specific genes that mediate developmental control (see Rubin 1985). However, the functional significance of associated large-scale structures, such as puffs, remains poorly understood.

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Authors and Affiliations

  1. The Carnegie Institute of Washington, 115 W. University Parkway, Baltimore, Maryland, 21210, USA

    A. Spradling

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  1. A. Spradling
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Editors and Affiliations

  1. Department of Genetics, Faculty of Sciences, University of Nijmegen, 6525 ED, Toernooiveld, Nijmegen, The Netherlands

    Wolfgang Hennig

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© 1987 Springer-Verlag Berlin Heidelberg

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Spradling, A. (1987). Gene Amplification in Dipteran Chromosomes. In: Hennig, W. (eds) Structure and Function of Eukaryotic Chromosomes. Results and Problems in Cell Differentiation, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-47783-9_12

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  • DOI: https://doi.org/10.1007/978-3-540-47783-9_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-22438-0

  • Online ISBN: 978-3-540-47783-9

  • eBook Packages: Springer Book Archive

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