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Bioimaging Metallomics

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Metallomics

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1055))

Abstract

This chapter focuses on bioimaging in metallomics, which involves metal and metalloids distribution in animal tissues. It starts with laser ablation-inductively coupled plasma-mass spectrometry followed by secondary ion mass spectrometry, synchrotron-based X-ray fluorescence, and electron microscopy, including transmission electron microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The basic principles of these techniques and their application for qualitative and quantitative imaging of elements are presented. Sample preparation for bioimaging is briefly discussed. The usefulness of element bioimaging is demonstrated for cells and several animal tissues, including the brain, liver, hair, eye, teeth, and bone. As such, this chapter addresses the state of the art in bioimaging metallomics.

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Abbreviations

2D:

Two dimensional

2D-PAGE:

Two-dimensional polyacrylamide gel electrophoresis

3D:

Three dimensional

AD:

Alzheimer disease

AmD:

Amyloid deposits

ATP:

Adenosine triphosphate

BNCT:

Boron neutron capture therapy

BSA:

Bovine serum albumin

CCD:

Charge-coupled device

CRM:

Certified reference material

CT:

Computed tomography

Da:

Dalton

DNA:

Deoxyribonucleic acid

DOTA:

Tetraazacyclododecane-1,4,7,10-tetraacetic acid

DTPABMA:

2-[bis[2-[carboxymethyl-[2-(methylamino)-2-oxoethyl]amino]ethyl]amino]acetic acid

EDX:

Energy-dispersive X-ray spectroscopy

EEL:

Energy loss

EELS:

Electron energy loss spectroscopy

EF:

Energy-filtered

EFTEM:

Energy-filtered transmission electron microscopy

EM:

Electron microscopy

ESI-MS:

Electrospray-mass spectrometry

FEG:

Field emission gun

FWHM:

Full width at half maximum

GE:

Gel electrophoresis

HPF:

High-pressure freezing

ICP:

Inductively coupled plasma

ICP-MS:

Inductively coupled plasma mass spectrometry

ID:

Isotope dilution

IEF-GE:

Isoelectric focusing gel electrophoresis

IPG:

Immobilized pH gradient strips

IR:

Infrared

IS:

Internal standard

LA:

Laser ablation

LA-ICP-MS:

Laser ablation-inductively coupled plasma mass spectrometry

LA-MS:

Laser ablation mass spectrometry

LMIG:

Liquid-metal ion gun

LOD:

Limit of detection

MC-ICP-MS:

Multicollector-inductively coupled plasma mass spectrometry

MeCAT:

Metal-coded affinity tag

METTEM:

Metal-tagging transmission electron microscopy

MLEM:

Maximum likelihood expectation maximization

MRI:

Magnetic resonance image

MSI:

Mass spectrometry imaging

ND-IEF-GE:

Non-denaturing isoelectric focusing gel electrophoresis

NINA:

Nanoimaging and nanoanalysis

NPs:

Nanoparticles

ODU:

Optical density unit

PAGE:

Polyacrylamide gel electrophoresis

QDs:

Quantum dots

QMS:

Quadrupole mass spectrometer

RAPTA:

Ru-arene bound to 1,3,5-triaza-7-phosphotricyclo-[3.3.1.1] decane

REEs:

Rare earth elements

ROI:

Regions of interest

RSF:

Relative sensitivity factor

SDS-PAGE:

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis

SEC-ICP-MS:

Size exclusion chromatography-inductively coupled plasma mass spectrometry

SEM:

Scanning electron microscopy

SF-ICP-MS:

Sector field-inductively coupled plasma mass spectrometry

SIMS:

Secondary ionization mass spectrometry

SN-ICP-MS:

Solution nebulization inductively coupled plasma mass spectrometry

SNR:

Signal-to-noise ratio

SOD:

Superoxide dismutase

SRXRF:

Synchrotron X-ray fluorescence

STEM:

Scanning transmission electron microscopy

STEM-EELS:

Scanning transmission electron microscopy-electron energy loss spectroscopy

TEM:

Transmission electron microscopy

ToF:

Time of flight

ToF-ICP-MS:

Time of flight-inductively coupled plasma mass spectrometry

ToF-SIMS:

Time of flight-secondary ionization mass spectrometry

UV:

Ultraviolet

UHV:

Ultra high vacuum

VSOP:

Very small iron oxide particles

WD:

Wilson’s disease

WDS:

Wavelength-dispersive spectrometry

XAFS:

X-ray absorption fine structure

XANES:

X-ray absorption near edge structure spectroscopy

XAS:

X-ray absorption spectroscopy

XRF:

X-ray fluorescence

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Authors and Affiliations

  1. Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

    Valderi Luiz Dressler & Edson Irineu Müller

  2. Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Dirce Pozebon

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  1. Valderi Luiz Dressler
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  2. Edson Irineu Müller
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  1. Universidade Estadual de Campinas – Unicamp, Institute of Chemistry, São Paulo, Brazil

    Marco Aurélio Zezzi Arruda

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Dressler, V.L., Müller, E.I., Pozebon, D. (2018). Bioimaging Metallomics. In: Arruda, M. (eds) Metallomics. Advances in Experimental Medicine and Biology(), vol 1055. Springer, Cham. https://doi.org/10.1007/978-3-319-90143-5_7

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