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Protocol for Somatic Embryogenesis in Passiflora cincinnata Mast. (Passifloraceae)

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Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 85))

Abstract

The process of somatic embryogenesis has become an essential asset, as it enables plant regeneration and large-scale propagation. Our research team has pioneered a reproducible protocol for somatic embryogenesis using mature zygotic embryo of P. cincinnata. Here, we describe in details the protocol for P. cincinnata, in which the explants were exposed to medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Due to the efficacy and reproducibility of this regeneration protocol, new perspectives arise as the protocol can be extended to other Passifloraceae species that arouse agronomic, ornamental and commercial interest. Moreover, this is an alternative for genetic transformation protocols, based up to now on the organogenic system.

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Acknowledgements

The Brazilian sponsoring agencies Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil; Grant no. CBB - APQ-01131-15) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil: Grant no. 459.529/2014-5) are acknowledged for the financial support. P.O.S. was recipient of a PhD scholarship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil). A.M.F. is currently recipient of a MSc scholarship from FAPEMIG, and L.M.V. a post-doctoral scholarship from CAPES/PNPD). Authors are also grateful to Dr. Daniela L. Paim-Pinto for generously making available Figs. 21.4B and 21.4C, and to Mr. Herculano José de Freitas, for kindly making available the photos in Fig. 21.1. Our gratitude to Caio G. Otoni for the English revision.

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos—LCT, Instituto de Biotecnologia Aplicada à Agropecuária-BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, 36570-900, Brazil

    Lorena Melo Vieira, Priscila Oliveira Silva, Amanda Mendes Fernandes & Wagner Campos Otoni

  2. Instituto de Biociências, Universidade Federal de Goiás, Regional Jataí, Jataí, GO, 75801-615, Brazil

    Diego Ismael Rocha

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  1. Lorena Melo Vieira
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  2. Priscila Oliveira Silva
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  3. Amanda Mendes Fernandes
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  4. Diego Ismael Rocha
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  5. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni .

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  1. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Shri Mohan Jain

  2. Federal Way, Washington, USA

    Pramod Gupta

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Vieira, L.M., Silva, P.O., Fernandes, A.M., Rocha, D.I., Otoni, W.C. (2018). Protocol for Somatic Embryogenesis in Passiflora cincinnata Mast. (Passifloraceae). In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-319-79087-9_21

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