Abstract
This chapter provides an overview of experiments conducted in space and on Earth using machines created to simulate microgravity. Today, research in space on the International Space Station (ISS) or in orbit, as well as the exploration by humans of extraterrestrial environments like the Moon or Mars, is of worldwide interest. The commercial use of space and future space tourism will further increase this interest. The space travels of European astronauts have contributed to this great success with their enormously positive PR activities before, during and after their respective missions.
In the past, space medicine and gravitational biology were disciplines familiar only to a small research community, but they are attracting a lot of interest today. A large number of exciting research findings have been discovered in the last 40 years. Today we know that microgravity has an enormous influence on the biology of human cells, in particular on cellular morphology, the cytoskeleton and growth behavior. Moreover, it changes various biological processes in human cells.
References
Adrian A, Schoppmann K, Sromicki J, Brungs S, von der Wiesche M, Hock B, et al. (2013) The oxidative burst reaction in mammalian cells depends on gravity. Cell Commun Signal 11:98
Aleshcheva G, Sahana J, Ma X, Hauslage J, Hemmersbach R, Egli M et al. (2013) Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine. PLoS One 8:e79057
Aleshcheva G, Wehland M, Sahana J, Bauer J, Corydon TJ, Hemmersbach R et al. (2015) Moderate alterations of the cytoskeleton in human chondrocytes after short-term microgravity produced by parabolic flight maneuvers could be prevented by up-regulation of BMP-2 and SOX-9. FASEB J 29:2303–2314
Aleshcheva G, Bauer J, Hemmersbach R, Slumstrup L, Wehland M, Infanger M et al. (2016) Scaffold-free tissue formation under real and simulated microgravity conditions. Basic Clin Pharmacol Toxicol 119(suppl 3):26–33
Battista N, Meloni MA, Bari M, Mastrangelo N, Galleri G, Rapino C et al. (2012) 5-Lipoxygenase-dependent apoptosis of human lymphocytes in the International Space Station: data from the ROALD experiment. FASEB J 26:1791–1798
Boonyaratanakornkit JB, Cogoli A, Li CF, Schopper T, Pippia P, Galleri G et al. (2005) Key gravity-sensitive signaling pathways drive T cell activation. FASEB J 19:2020–2022
Chang TT, Walther I, Li CF, Boonyaratanakornkit J, Galleri G, Meloni MA et al. (2012) The Rel/NF-kappaB pathway and transcription of immediate early genes in T cell activation are inhibited by microgravity. J Leukoc Biol 92:1133–1145
Clejan S, O’Connor K, Rosensweig N (2001) Tri-dimensional prostate cell cultures in simulated microgravity and induced changes in lipid second messengers and signal transduction. J Cell Mol Med 5:60–73
Cogoli A, Tschopp A (1985) Lymphocyte reactivity during spaceflight. Immunol Today 6:1–4
Cogoli A, Valluchi-Morf M, Bohringer HR, Vanni MR, Muller M (1979) Effect of gravity on lymphocyte proliferation. Life Sci Space Res 17:219–224
Cogoli A, Tschopp A, Fuchs-Bislin P (1984) Cell sensitivity to gravity. Science 225:228–230
Cogoli-Greuter M (1998) Influence of microgravity on mitogen binding, motility and cytoskeleton patterns of T lymphocytes and Jurkat cells-experiments on sounding rockets. Jpn J Aerospace Environ Med 35:27–39
Cogoli-Greuter M, Lovis P, Vadrucci S (2004) Signal transduction in T cells: an overview. J Gravit Physiol 11:53–56
Corydon TJ, Kopp S, Wehland M, Braun M, Schutte A, Mayer T et al (2016b) Alterations of the cytoskeleton in human cells in space proved by life-cell imaging. Sci Rep 6:20043
Corydon TJ, Mann V, Slumstrup L, Kopp S, Sahana J, Askou AL et al (2016a) Reduced expression of cytoskeletal and extracellular matrix genes in human adult retinal pigment epithelium cells exposed to simulated microgravity. Cell Physiol Biochem 40:1–17
Dai Z, Wu F, Chen J, Xu H, Wang H, Guo F et al. (2013) Actin microfilament mediates osteoblast Cbfa1 responsiveness to BMP2 under simulated microgravity. PLoS One 8:e63661
Galleri G, Meloni MA, Camboni MG, Deligios M, Cogoli A, Pippia P (2002) Signal transduction in T lymphocites under simulated microgravity conditions: involvement of PKC isoforms. J Gravit Physiol 9:289–290
Gasperi V, Rapino C, Battista N, Bari M, Mastrangelo N, Angeletti S et al. (2014) A functional interplay between 5-lipoxygenase and mu-calpain affects survival and cytokine profile of human Jurkat T lymphocyte exposed to simulated microgravity. Biomed Res Int 2014:782390
Grimm D, Bauer J, Kossmehl P, Shakibaei M, Schoberger J, Pickenhahn H et al. (2002) Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells. FASEB J 16(6):604–606
Grimm D, Infanger M, Westphal K, Ulbrich C, Pietsch J, Kossmehl P et al. (2009) A delayed type of three-dimensional growth of human endothelial cells under simulated weightlessness. Tissue Eng Part A 15:2267–2275
Grimm D, Bauer J, Ulbrich C, Westphal K, Wehland M, Infanger M et al. (2010) Different responsiveness of endothelial cells to vascular endothelial growth factor and basic v growth factor added to culture media under gravity and simulated microgravity. Tissue Eng Part A 16:1559–1573
Grimm D, Wise P, Lebert M, Richter P, Baatout S (2011) How and why does the proteome respond to microgravity? Expert Rev Proteomics 8:13–27
Grimm D, Wehland M, Pietsch J, Aleshcheva G, Wise P, van Loon J et al. (2014) Growing tissues in real and simulated microgravity: new methods for tissue engineering. Tissue Eng Part B Rev 20:555–566
Grimm D, Grosse J, Wehland M, Mann V, Reseland JE, Sundaresan A, Corydon TJ (2016) The impact of microgravity on bone in humans. Bone 87:44–56
Grosse J, Wehland M, Pietsch J, Ma X, Ulbrich C, Schulz H et al. (2012a) Short-term weightlessness produced by parabolic flight maneuvers altered gene expression patterns in human endothelial cells. FASEB J 26:639–655
Grosse J, Wehland M, Pietsch J, Schulz H, Saar K, Hubner N et al. (2012b) Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. FASEB J 26:5124–5140
Hatton JP, Gaubert F, Lewis ML, Darsel Y, Ohlmann P, Cazenave JP et al. (1999) The kinetics of translocation and cellular quantity of protein kinase C in human leukocytes are modified during spaceflight. FASEB J 13(suppl):S23–S33
Hughes-Fulford M (2003) Function of the cytoskeleton in gravisensing during spaceflight. Adv Space Res 32:1585–1593
Infanger M, Kossmehl P, Shakibaei M, Baatout S, Witzing A, Grosse J et al. (2006) Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: impact of vascular endothelial growth factor. Apoptosis 11:749–764
Ingber D (1999) How cells (might) sense microgravity. FASEB J 13(suppl):S3–15
Ingram M, Techy GB, Saroufeem R, Yazan O, Narayan KS, Goodwin TJ et al. (1997) Three-dimensional growth patterns of various human tumor cell lines in simulated microgravity of a NASA bioreactor. In Vitro Cell Dev Biol Anim 33:459–466
Jackson A, Vayssiere B, Garcia T, Newell W, Baron R, Roman-Roman S et al. (2005) Gene array analysis of Wnt-regulated genes in C3H10T1/2 cells. Bone 36:585–598
Kang CY, Zou L, Yuan M, Wang Y, Li TZ, Zhang Y et al. (2011) Impact of simulated microgravity on microvascular endothelial cell apoptosis. Eur J Appl Physiol 111:2131–2138
Kopp S, Warnke E, Wehland M, Aleshcheva G, Magnusson NE, Hemmersbach R et al. (2015) Mechanisms of three-dimensional growth of thyroid cells during long-term simulated microgravity. Sci Rep 5:16691
Kumei Y, Morita S, Katano H, Akiyama H, Hirano M, Oyha K et al. (2006) Microgravity signal ensnarls cell adhesion, cytoskeleton, and matrix proteins of rat osteoblasts: osteopontin, CD44, osteonectin, and alpha-tubulin. Ann N Y Acad Sci 1090:311–317
Lewis ML, Reynolds JL, Cubano LA, Hatton JP, Lawless BD, Piepmeier EH (1998) Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat). FASEB J 12:1007–1018
Ma X, Sickmann A, Pietsch J, Wildgruber R, Weber G, Infanger M et al. (2014a) Proteomic differences between microvascular endothelial cells and the EA.hy926 cell line forming three-dimensional structures. Proteomics 14:689–698
Ma X, Pietsch J, Wehland M, Schulz H, Saar K, Hubner N et al. (2014b) Differential gene expression profile and altered cytokine secretion of thyroid cancer cells in space. FASEB J 28:813–835
Maccarrone M, Battista N, Meloni M, Bari M, Galleri G, Pippia P et al. (2003) Creating conditions similar to those that occur during exposure of cells to microgravity induces apoptosis in human lymphocytes by 5-lipoxygenase-mediated mitochondrial uncoupling and cytochrome c release. J Leukoc Biol 73:472–481
Mader TH, Gibson CR, Pass AF, Kramer LA, Lee AG, Fogarty J et al. (2011) Optic disc edema, globe flattening, choroidal folds, and hyperopic shifts observed in astronauts after long-duration space flight. Ophthalmology 118:2058–2069
Mader TH, Gibson CR, Lee AG (2016) Choroidal folds in astronauts. Invest Ophthalmol Vis Sci 57:592
Morrow MA (2006) Clinorotation differentially inhibits T-lymphocyte transcription factor activation. In Vitro Cell Dev Biol Anim 42:153–158
Nabavi N, Khandani A, Camirand A, Harrison RE (2011) Effects of microgravity on osteoclast bone resorption and osteoblast cytoskeletal organization and adhesion. Bone 49:965–974
Niehoff A, Brüggemann GP, Zaucke F, Eckstein F, Bloch W, Mündermann A et al. (2016) Long-duration space flight and cartilage adaptation: first results on changes in tissue metabolism. Osteoarthr Cartil 24:S144–S145
Pietsch J, Bauer J, Egli M, Infanger M, Wise P, Ulbrich C et al. (2011) The effects of weightlessness on the human organism and mammalian cells. Curr Mol Med 11:350–364
Pietsch J, Ma X, Wehland M, Aleshcheva G, Schwarzwalder A, Segerer J et al. (2013) Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission. Biomaterials 34:7694–7705
Rijken PJ, de Groot RP, Briegleb W, Kruijer W, Verkleij AJ, Boonstra J et al. (1991a) Epidermal growth factor-induced cell rounding is sensitive to simulated microgravity. Aviat Space Environ Med 62:32–36
Rijken PJ, Hage WJ, van Bergen en Henegouwen PM, Verkleij AJ, Boonstra J (1991b) Epidermal growth factor induces rapid reorganization of the actin microfilament system in human A431 cells. J Cell Sci 100(Pt 3):491–499
Shi F, Zhao TZ, Wang YC, Cao XS, Yang CB, Gao Y et al. (2016) The impact of simulated weightlessness on endothelium-dependent angiogenesis and the role of caveolae/caveolin-1. Cell Physiol Biochem 38:502–513
Spatz JM, Wein MN, Gooi JH, Qu Y, Garr JL, Liu S et al. (2015) The Wnt inhibitor sclerostin is up-regulated by mechanical unloading in osteocytes in vitro. J Biol Chem 290:16744–16758
Stamenkovic V, Keller G, Nesic D, Cogoli A, Grogan SP (2010) Neocartilage formation in 1 g, simulated, and microgravity environments: implications for tissue engineering. Tissue Eng Part A 16:1729–1736
Svejgaard B, Wehland M, Ma X, Kopp S, Sahana J, Warnke E et al. (2015) Common effects on cancer cells exerted by a random positioning machine and a 2d clinostat. PLoS One 10:e0135157
Tauber S, Hauschild S, Paulsen K, Gutewort A, Raig C, Hurlimann E et al. (2015) Signal transduction in primary human T lymphocytes in altered gravity during parabolic flight and clinostat experiments. Cell Physiol Biochem 35:1034–1051
Thiel CS, Paulsen K, Bradacs G, Lust K, Tauber S, Dumrese C et al. (2012) Rapid alterations of cell cycle control proteins in human T lymphocytes in microgravity. Cell Commun Signal 10:1
Ulbrich C, Westphal K, Pietsch J, Winkler HD, Leder A, Bauer J et al. (2010) Characterization of human chondrocytes exposed to simulated microgravity. Cell Physiol Biochem 25:551–560
Ulbrich C, Pietsch J, Grosse J, Wehland M, Schulz H, Saar K et al. (2011) Differential gene regulation under altered gravity conditions in follicular thyroid cancer cells: relationship between the extracellular matrix and the cytoskeleton. Cell Physiol Biochem 28:185–198
Vassy J, Portet S, Beil M, Millot G, Fauvel-Lafeve F, Karniguian A et al. (2001) The effect of weightlessness on cytoskeleton architecture and proliferation of human breast cancer cell line MCF-7. FASEB J 15:1104–1106
Versari S, Longinotti G, Barenghi L, Maier JA, Bradamante S (2013) The challenging environment on board the International Space Station affects endothelial cell function by triggering oxidative stress through thioredoxin interacting protein overexpression: the ESA-SPHINX experiment. FASEB J 27:4466–4475
Vorselen D, Roos WH, MacKintosh FC, Wuite GJ, van Loon JJ (2014) The role of the cytoskeleton in sensing changes in gravity by nonspecialized cells. FASEB J 28:536–547
Warnke E, Pietsch J, Wehland M, Bauer J, Infanger M, Gorog M et al. (2014) Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1. Cell Commun Signal 12:32
Wehland M, Aleshcheva G, Schulz H, Saar K, Hubner N, Hemmersbach Ret al. (2015) Differential gene expression of human chondrocytes cultured under short-term altered gravity conditions during parabolic flight maneuvers. Cell Commun Signal 13:18
White RJ, Averner M (2001) Humans in space. Nature 409:1115–1118
Yang X, Sun L-W, Liang M, Wang X-N, Fan Y-B (2015) The response of wnt/ß-catenin signaling pathway in osteocytes under simulated microgravity. Microgravity Sci Tech 27:473–483
Zayzafoon M, Meyers VE, McDonald JM (2005) Microgravity: the immune response and bone. Immunol Rev 208:267–280
Zhang Y, Lau P, Pansky A, Kassack M, Hemmersbach R, Tobiasch E (2014) The influence of simulated microgravity on purinergic signaling is different between individual culture and endothelial and smooth muscle cell coculture. Biomed Res Int 2014:413708
Zwart SR, Gregory JF, Zeisel SH, Gibson CR, Mader TH, Kinchen JM et al. (2016) Genotype, B-vitamin status, and androgens affect spaceflight-induced ophthalmic changes. FASEB J 30:141–148
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Grimm, D. (2017). Cell Biology in Space. In: Biotechnology in Space. SpringerBriefs in Space Life Sciences. Springer, Cham. https://doi.org/10.1007/978-3-319-64054-9_5
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DOI: https://doi.org/10.1007/978-3-319-64054-9_5
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