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Myosin and Actin Filaments in Muscle: Structures and Interactions

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Fibrous Proteins: Structures and Mechanisms

Part of the book series: Subcellular Biochemistry ((SCBI,volume 82))

Abstract

In the last decade, improvements in electron microscopy and image processing have permitted significantly higher resolutions to be achieved (sometimes <1 nm) when studying isolated actin and myosin filaments. In the case of actin filaments the changing structure when troponin binds calcium ions can be followed using electron microscopy and single particle analysis to reveal what happens on each of the seven non-equivalent pseudo-repeats of the tropomyosin α-helical coiled-coil. In the case of the known family of myosin filaments not only are the myosin head arrangements under relaxing conditions being defined, but the latest analysis, also using single particle methods, is starting to reveal the way that the α-helical coiled-coil myosin rods are packed to give the filament backbones.

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Acknowledgements

Much of our work reported here has been funded by the British Heart Foundation, with earlier work supported by the UK Medical Research Council and the Wellcome Trust. We are indebted to all these funding agencies. JMS and DP are currently funded on the BHF Fellowship grant (FS/14/18/3071). EM is funded by Cancer Research UK (grant C12209/A16749). We are indebted to Dr. John Wray for helpful comments about crustacean myosin filaments.

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  1. Muscle Contraction Group, School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, BS8 1TD, UK

    John M. Squire & Danielle M. Paul

  2. Division of Structural Biology, The Institute of Cancer Research, London, SW3 6JB, UK

    Edward P. Morris

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  1. John M. Squire
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Correspondence to John M. Squire .

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  1. Institute of Fundamental Sciences and Riddet Institute, Massey University, Palmerston North, New Zealand

    David A.D. Parry

  2. Muscle Contraction Group, School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, United Kingdom

    John M. Squire

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Squire, J.M., Paul, D.M., Morris, E.P. (2017). Myosin and Actin Filaments in Muscle: Structures and Interactions. In: Parry, D., Squire, J. (eds) Fibrous Proteins: Structures and Mechanisms. Subcellular Biochemistry, vol 82. Springer, Cham. https://doi.org/10.1007/978-3-319-49674-0_11

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