Abstract
Myosin-binding protein-C (MyBP-C) is an accessory protein of the myosin filaments of vertebrate striated muscle. In the heart, it plays a key role in modulating contractility in response to β-adrenergic stimulation. Mutations in the cardiac isoform (cMyBP-C) are a leading cause of inherited hypertrophic cardiomyopathy. Understanding cMyBP-C function and its role in disease requires knowledge of the structure of the molecule, its organization in the sarcomere, and its interactions with other sarcomeric proteins. Here we review the main structural features of this modular, elongated molecule and the properties of some of its key domains. We describe observations suggesting that the bulk of the molecule extends perpendicular to the thick filament, enabling it to reach neighboring thin filaments in the sarcomere. We review structural and functional evidence for interaction of its N-terminal domains with actin and how this may modulate thin filament activation. We also discuss the effects that phosphorylation of cMyBP-C has on some of these structural features and how this might relate to cMyBP-C function in the beating heart.
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Ababou A, Gautel M, Pfuhl M (2007) Dissecting the N-terminal myosin binding site of human cardiac myosin-binding protein C. Structure and myosin binding of domain C2. J Biol Chem 282:9204–9215
Al-Khayat HA, Kensler RW, Squire JM, Marston SB, Morris EP (2013) Atomic model of the human cardiac muscle myosin filament. Proc Natl Acad Sci U S A 110:318–323
Barefield D, Sadayappan S (2010) Phosphorylation and function of cardiac myosin binding protein-C in health and disease. J Mol Cell Cardiol 48:866–875
Bennett P, Craig R, Starr R, Offer G (1986) The ultrastructural location of C-protein, X-protein and H-protein in rabbit muscle. J Muscle Res Cell Motil 7:550–567
Bennett PM, Furst DO, Gautel M (1999) The C-protein (myosin binding protein C) family: regulators of contraction and sarcomere formation? Rev Physiol Biochem Pharmacol 138:203–234
Bhuiyan MS, Gulick J, Osinska H, Gupta M, Robbins J (2012) Determination of the critical residues responsible for cardiac myosin binding protein C’s interactions. J Mol Cell Cardiol 53:838–847
Craig R (1977) Structure of A-segments from frog and rabbit skeletal muscle. J Mol Biol 109:69–81
Craig R, Offer G (1976) The location of C-protein in rabbit skeletal muscle. Proc R Soc Lond B Biol Sci 192:451–461
Flashman E, Redwood C, Moolman-Smook J, Watkins H (2004) Cardiac myosin binding protein C: its role in physiology and disease. Circ Res 94:1279–1289
Govada L, Carpenter L, da Fonseca PC, Helliwell JR, Rizkallah P, Flashman E, Chayen NE, Redwood C, Squire JM (2008) Crystal structure of the C1 domain of cardiac myosin binding protein-C: implications for hypertrophic cardiomyopathy. J Mol Biol 378:387–397
Gregorio CC, Granzier H, Sorimachi H, Labeit S (1999) Muscle assembly: a titanic achievement? Curr Opin Cell Biol 11:18–25
Gruen M, Gautel M (1999) Mutations in beta-myosin S2 that cause familial hypertrophic cardiomyopathy (FHC) abolish the interaction with the regulatory domain of myosin-binding protein-C. J Mol Biol 286:933–949
Gruen M, Prinz H, Gautel M (1999) cAPK-phosphorylation controls the interaction of the regulatory domain of cardiac myosin binding protein C with myosin-S2 in an on–off fashion. FEBS Lett 453:254–259
Hanson J, O’Brien EJ, Bennett PM (1971) Structure of the myosin-containing filament assembly (A-segment) separated from frog skeletal muscle. J Mol Biol 58:865–871
Harris SP, Lyons RG, Bezold KL (2011) In the thick of it: HCM-causing mutations in myosin binding proteins of the thick filament. Circ Res 108:751–764
Howarth JW, Ramisetti S, Nolan K, Sadayappan S, Rosevear PR (2012) Structural insight into unique cardiac myosin-binding protein-C motif: a partially folded domain. J Biol Chem 287:8254–8262
Jeffries CM, Whitten AE, Harris SP, Trewhella J (2008) Small-angle X-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein C. J Mol Biol 377:1186–1199
Karsai A, Kellermayer MS, Harris SP (2011) Mechanical unfolding of cardiac myosin binding protein-C by atomic force microscopy. Biophys J 101:1968–1977
Lee KH, Sadayappan S, Harris S, Craig R (2013) Determination of MyBP-C orientation in the cardiac sarcomere by immuno-EM. Biophys J 104:309a
Luther PK, Bennett PM, Knupp C, Craig R, Padron R, Harris SP, Patel J, Moss RL (2008) Understanding the organisation and role of myosin binding protein C in normal striated muscle by comparison with MyBP-C knockout cardiac muscle. J Mol Biol 384:60–72
Luther PK, Craig R (2011) Modulation of striated muscle contraction by binding of myosin binding protein C to actin. Bioarchitecture 1:277–283
Luther PK, Winkler H, Taylor K, Zoghbi ME, Craig R, Padron R, Squire JM, Liu J (2011) Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle. Proc Natl Acad Sci U S A 108:11423–11428
Michalek AJ, Howarth JW, Gulick J, Previs MJ, Robbins J, Rosevear PR, Warshaw DM (2013) Phosphorylation modulates the mechanical stability of the cardiac myosin-binding protein C motif. Biophys J 104:442–452
Moolman-Smook J, Flashman E, de Lange W, Li Z, Corfield V, Redwood C, Watkins H (2002) Identification of novel interactions between domains of myosin binding protein-C that are modulated by hypertrophic cardiomyopathy missense mutations. Circ Res 91:704–711
Moos C, Mason CM, Besterman JM, Feng IN, Dubin JH (1978) The binding of skeletal muscle C-protein to F-actin, and its relation to the interaction of actin with myosin subfragment-1. J Mol Biol 124:571–586
Mun JY, Gulick J, Robbins J, Woodhead J, Lehman W, Craig R (2011) Electron microscopy and 3D reconstruction of F-actin decorated with cardiac myosin-binding protein C (cMyBP-C). J Mol Biol 410:214–225
Mun JY, Previs MJ, Yu H, Gulick J, Tobacman LS, Previs SB, Robbins J, Warshaw DM, Craig R (2013) Myosin-binding protein C displaces tropomyosin to activate cardiac thin filaments and governs their speed by an independent mechanism. Proc Natl Acad Sci U S A. In press.
Offer G, Moos C, Starr R (1973) A new protein of the thick filaments of vertebrate skeletal myofibrils. Extraction, purification and characterization. J Mol Biol 74:653–676
Orlova A, Galkin VE, Jeffries CM, Egelman EH, Trewhella J (2011) The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin. J Mol Biol 412:379–386
Previs MJ, Beck PS, Gulick J, Robbins J, Warshaw DM (2012) Molecular mechanics of cardiac myosin-binding protein C in native thick filaments. Science 337:1215–1218
Previs MJ, Yu H, Beck PS, Gulick J, Robbins J, Warshaw DM (2013) Dephosphorylated cardiac myosin-binding protein c (cMyBP-C) activates native cardiac thin filaments within the C-zone of native cardiac thick filaments. Biophys J 104:186a
Ratti J, Rostkova E, Gautel M, Pfuhl M (2011) Structure and interactions of myosin-binding protein C domain C0: cardiac-specific regulation of myosin at its neck? J Biol Chem 286:12650–12658
Razumova MV, Shaffer JF, Tu AY, Flint GV, Regnier M, Harris SP (2006) Effects of the N-terminal domains of myosin binding protein-C in an in vitro motility assay: evidence for long-lived cross-bridges. J Biol Chem 281:35846–35854
Rybakova IN, Greaser ML, Moss RL (2011) Myosin binding protein C interaction with actin: characterization and mapping of the binding site. J Biol Chem 286:2008–2016
Saber W, Begin KJ, Warshaw DM, VanBuren P (2008) Cardiac myosin binding protein-C modulates actomyosin binding and kinetics in the in vitro motility assay. J Mol Cell Cardiol 44:1053–1061
Shaffer JF, Harris SP (2009) Species-specific differences in the Pro-Ala rich region of cardiac myosin binding protein-C. J Muscle Res Cell Motil 30:303–306
Shaffer JF, Kensler RW, Harris SP (2009) The myosin-binding protein C motif binds to F-actin in a phosphorylation-sensitive manner. J Biol Chem 284:12318–12327
Squire JM, Luther PK, Knupp C (2003) Structural evidence for the interaction of C-protein (MyBP-C) with actin and sequence identification of a possible actin-binding domain. J Mol Biol 331:713–724
Weith A, Sadayappan S, Gulick J, Previs MJ, VanBuren P, Robbins J, Warshaw DM (2012) Unique single molecule binding of cardiac myosin binding protein-C to actin and phosphorylation-dependent inhibition of actomyosin motility requires 17 amino acids of the motif domain. J Mol Cell Cardiol 52:219–227
Weith AE, Previs MJ, Hoeprich GJ, Previs SB, Gulick J, Robbins J, Warshaw DM (2012) The extent of cardiac myosin binding protein-C phosphorylation modulates actomyosin function in a graded manner. J Muscle Res Cell Motil 33:449–459
White HD, Belknap B, Harris SP (2013) Activation and inhibition of F-actin and cardiac thin filaments by the N-terminal domains of cardiac myosin binding protein C. Biophys J 104:158a–159a
White HD, Harris S (2012) Activation of cardiac thin filaments by N-terminal domains of cardiac myosin binding protein C. Biophys J 102:435a
Whitten AE, Jeffries CM, Harris SP, Trewhella J (2008) Cardiac myosin-binding protein C decorates F-actin: implications for cardiac function. Proc Natl Acad Sci U S A 105:18360–18365
Yamamoto K (1986) The binding of skeletal muscle C-protein to regulated actin. FEBS Lett 208:123–127
Zoghbi ME, Woodhead JL, Moss RL, Craig R (2008) Three-dimensional structure of vertebrate cardiac muscle myosin filaments. Proc Natl Acad Sci U S A 105:2386–2390
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This work was supported by National Institutes of Health grants R01 AR034711 and P01 HL059408 and by British Heart Foundation Programme Grant RG/11/21/29335.
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Craig, R., Lee, K.H., Mun, J.Y. et al. Structure, sarcomeric organization, and thin filament binding of cardiac myosin-binding protein-C. Pflugers Arch - Eur J Physiol 466, 425–431 (2014). https://doi.org/10.1007/s00424-013-1426-6
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DOI: https://doi.org/10.1007/s00424-013-1426-6