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Analytical Techniques to Assess Medicinal Plants Value Addition After Microbial Associations

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Modern Tools and Techniques to Understand Microbes

Abstract

In the high-throughput era of science, technologies like high-performance liquid chromatography (HPLC) are growing in importance for their accurate and precise analysis of chemicals and drugs of diverse nature. It is used widely in the pharmaceutical, medicinal, and aromatic plant industry for the isolation and purification of phytomolecules of therapeutic and commercial interest. Due to the gradual increase in the demand for phytochemicals in food and pharmaceutical industries, the importance of process-scale HPLC as a purification tool has been increasing. The most challenging and thrilling field of recent scientific research is the in vitro production of plant secondary metabolites. Therefore, in order to supply required phytochemicals, the in vitro protocol for the establishment of plant culture and production of phytochemicals has to be carefully monitored. In this chapter, the HPLC analysis of secondary metabolite content of an important medicinal plant Aloe vera L. has been performed under unstressed and salt-stressed environment. The secondary metabolite content has also been compared upon inoculation of A. vera with a symbiotic endophytic fungus Piriformospora indica.

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Acknowledgments

Authors are thankful to University Grant Commission, Government of India, for providing financial assistance during the course of the study. Authors are thankful to Jamia Hamdard for providing the HPLC facility.

Ajit Varma is thankful to Department of Science and Technology and Department of Biotechnology for partial financial funding and to DST-FIST for providing confocal microscope facility.

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Authors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Block E-3, 4th Floor, Sector 125, Noida, 201303, India

    Priyanka Sharma, Hemesh Joshi, Amit C Kharkwal & Ajit Varma

  2. JamiaHamdard University, Hamdard Nagar, New Delhi, 110062, India

    MZ Abdin

Authors
  1. Priyanka Sharma
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  2. Hemesh Joshi
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  3. MZ Abdin
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  4. Amit C Kharkwal
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  5. Ajit Varma
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Corresponding author

Correspondence to Amit C Kharkwal .

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Editors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Ajit Varma

  2. ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Kusmaur, Mau Nath Bhanjan, Uttar Pradesh, India

    Arun Kumar Sharma

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Sharma, P., Joshi, H., Abdin, M., Kharkwal, A.C., Varma, A. (2017). Analytical Techniques to Assess Medicinal Plants Value Addition After Microbial Associations. In: Varma, A., Sharma, A. (eds) Modern Tools and Techniques to Understand Microbes. Springer, Cham. https://doi.org/10.1007/978-3-319-49197-4_17

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