Abstract
Axillary bud development and adventitious bud formation was obtained with decapitated shoot explants of Aloe barbadensis Mill. Maximal bud growth and rooting of shoots was obtained on a modified medium of Murashige and Skoog supplemented with 5 μM IBA. More adventitious and axillary buds developed on nutrient media supplemented with IBA than with NAA. Axillary buds but not adventitious buds developed with IAA in the medium. Morphogenesis was inhibited by 2,4-D. Kinetin, benzyladenine and thidiazuron were toxic to the explants and did not stimulate the development of axillary of adventitious buds. The optimal temperature for bud growth and development was 25°C. Axillary bud growth and the formation of adventitious buds was slowed down at 10°C and totally inhibited by 30°C. The optimal sucrose concentration was 3% with the inhibition of bud growth and development by higher sucrose levels.
Similar content being viewed by others
References
Groenewald EG, Koeleman A & Wessels DCJ (1975) Callus formation and plant regeneration from seed tissue of Aloe pretoriensis Pole Evans. Z. Pflanzenphysiol. 75: 270–272
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Natali L, Sanchez IC & Cavallini A (1990) In vitro culture of Aloe barbadensis Mill: Micropropagation from vegetative meristems. Plant Cell Tiss. Org. Cult. 20: 71–74
Reynolds GW (1966) The Aloes of Tropical Africa and Madagascar. The Trustees of the Aloes Book Fund, Mbabane
Snedecor GW & Cochran WG (1972) Statistical Methods. 6th edition (pp 258–298). Iowa State University Press, Iowa.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meyer, H.J., van Staden, J. Rapid in vitro propagation of Aloe barbadensis Mill. Plant Cell Tiss Organ Cult 26, 167–171 (1991). https://doi.org/10.1007/BF00039939
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00039939