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Optical Coherence Microscopy

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Optical Coherence Tomography

Abstract

Cellular imaging of human tissues remains an important advance for many clinical applications of optical coherence tomography (OCT). Imaging cells with traditional OCT systems has not been possible due to the limited transverse resolution of such techniques. Optical coherence microscopy (OCM) refers to OCT methods that achieve high transverse resolution to visualize cells and subcellular features. This chapter provides a comprehensive discussion of the rationale for cellular imaging in human tissues as well as a review of the key technological advances required to achieve it. Time domain and Fourier domain OCM approaches are described with an emphasis on state of the art system designs, including miniaturized endoscopic imaging probes. Clinical applications are discussed and multiple examples of cellular imaging in human tissues are provided.

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Acknowledgments

We would like to acknowledge scientific discussions and contributions from Drs. Yu Chen, James Connolly, Shu-Wei Huang, Robert Huber, Desmond Adler, Norihiko Nishizawa, Joseph Schmitt. This research was sponsored in part by the National Institutes of Health R01-CA75289, R01-EY11289, and R01-CA178636; the Air Force Office of Scientific Research FA9550-040-1-0011 and F∼9550-12-1-0499.

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Authors and Affiliations

  1. Massachusetts General Hospital, Boston, MA, USA

    Aaron D. Aguirre

  2. Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Aaron D. Aguirre, Chao Zhou, Hsiang-Chieh Lee, Osman O. Ahsen & James G. Fujimoto

  3. Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA, 18015, USA

    Chao Zhou

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  1. Aaron D. Aguirre
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  5. James G. Fujimoto
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Correspondence to Aaron D. Aguirre .

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  1. Center for Medical Physics and Biomedical Engineering, Medical University Vienna, General Hospital Vienna, Vienna, Austria

    Wolfgang Drexler

  2. Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    James G. Fujimoto

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Aguirre, A.D., Zhou, C., Lee, HC., Ahsen, O.O., Fujimoto, J.G. (2015). Optical Coherence Microscopy. In: Drexler, W., Fujimoto, J. (eds) Optical Coherence Tomography. Springer, Cham. https://doi.org/10.1007/978-3-319-06419-2_29

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