Summary
This chapter covers the use of dithiocarbamate derivatives chelating ferrous iron (Fe) as spin trap reagents for detection of nitric oxide (·NO) produced in vivo in small laboratory animals by electron paramagnetic resonance (EPR) spectroscopy. The principle of this method is based on the high affinity of ·NO toward dithiocarbamate-Fe complexes, forming stable nitrosyl-Fe-dithiocarbamate complexes whose characteristic three-line spectrum with giso=2.04 can readily be detected by EPR spectroscopy. Experiments in our laboratory showed that among several dithiocarbamate derivatives, N-methyl-D-glucamin dithiocarbamate, which is highly water-soluble and relatively non-toxic, is the most promising derivative for studying the production of ·NO in vivo in physiological as well as pathophysiological conditions where ·NO is overproduced.
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© 1995 Birkhäuser Verlag
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Lai, CS., Komarov, A.M. (1995). Dithiocarbamate spin traps for in vivo detection of nitric oxide production in mice. In: Ohya-Nishiguchi, H., Packer, L. (eds) Bioradicals Detected by ESR Spectroscopy. MCBU Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9059-5_12
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DOI: https://doi.org/10.1007/978-3-0348-9059-5_12
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