Abstract
Exposure of corn seedlings to high concentrations of Cd ions induces formation of large quantities of polypeptidic compounds which are composed almost exclusively of Glu, Cys and Gly and which bind Cd through thiolate coordination. These polymers occur in classes with molecular weights of 4000 and 8000, respectively, and are made up of chains of about 2000 crosslinked by disulfide bridges. By derivatization and HPLC separation four different molecular species of similar amino acid composition were resolved. In the “native” state all Cys are either coordinated to Cd or linked in intra-and intermolecular disulfide bridges. The overall thiol-to-Cd ratio is close to 2. Judged from the refractoriness to endopeptidase digestion it is inferred that as in glutathione Glu is linked to the adjoining amino acid through its γ-carboxyl group.
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References
De Jong, C., Hughes, G.J., van Wieringen, E. and Wilson, K.J. (1982) J. Chromatogr., 241, 345–359
Dupont, J.C., Casale, G. and Kirchmann, R. (1980) Int. J. Environ. Stud. 15, 33–40
Grill, E. (1987) This volume, pp. 317–322
Hoagland, D.R. and Arnon, D.I. (1950) Calif. Agric. Exp. Stn. Circ. No. 347
Jackson, P.J., Roth, E.J., McClure, P.R. and Naranjo, C.M. (1984) Plant Physiol. 75, 914–918
Hogan, G.D. and Rauser W.E. (1981) J. Exp. Bot. 32, 27–36
Murasugi, A., Wada, C. and Hayashi, Y. (1981) J. Biochem. 90, 1561–1564
Rauser, W.E., Hartmann, H.-J. and Weser, U. (1983) FEBS Lett. 164, 102–104
Robinson, N.J., Barton, K., Naranjo, C.M., Sillerud, L.O., Trewhella, J., Watt, K. and Jackson, P.J. (1986) This volume, pp. 323–327
Wagner, G.J. and Trotter, M.M. (1982) Plant Physiol. 69, 804–809
Rauser, W.E. and Glover, J. (1984) Can. J. Bot. 62, 1645–1650
Van Driessche, E., Beekmans, S., Dejaegere, R., and Kanarek, L. (1984) Anal. Biochem., 141, 184–188
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© 1987 Springer Basel AG
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Bernhard, W.R., Kägi, J.H.R. (1987). Purification and Characterization of Atypical Cadmium-Binding Polypeptides from Zea Mays. In: Kägi, J.H.R., Kojima, Y. (eds) Metallothionein II. Experientia Supplementum, vol 52. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6784-9_27
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DOI: https://doi.org/10.1007/978-3-0348-6784-9_27
Publisher Name: Birkhäuser, Basel
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