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Microbial Metabolites Annotation by Mass Spectrometry-Based Metabolomics

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Microbial Natural Products Chemistry

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1439))

Abstract

Since the discovery of penicillin, microbial metabolites have been extensively investigated for drug discovery purposes. In the last decades, microbial derived compounds have gained increasing attention in different fields from pharmacognosy to industry and agriculture. Microbial metabolites in microbiomes present specific functions and can be associated with the maintenance of the natural ecosystems. These metabolites may exhibit a broad range of biological activities of great interest to human purposes. Samples from either microbial isolated cultures or microbiomes consist of complex mixtures of metabolites and their analysis are not a simple process. Mass spectrometry-based metabolomics encompass a set of analytical methods that have brought several improvements to the microbial natural products field. This analytical tool allows the comprehensively detection of metabolites, and therefore, the access of the chemical profile from those biological samples. These analyses generate thousands of mass spectra which is challenging to analyse. In this context, bioinformatic metabolomics tools have been successfully employed to accelerate and facilitate the investigation of specialized microbial metabolites. Herein, we describe metabolomics tools used to provide chemical information for the metabolites, and furthermore, we discuss how they can improve investigation of microbial cultures and interactions.

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  1. Authors Paulo Wender P. Gomes, Talita Carla de Tralia Medeiros and Naydja Moralles Maimone have equally contributed to this chapter.

Authors and Affiliations

  1. Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA

    Paulo Wender P. Gomes & Anelize Bauermeister

  2. Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil

    Talita Carla de Tralia Medeiros & Luiz Alberto Beraldo de Moraes

  3. Departamento de Ciências Exatas, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo, Piracicaba, São Paulo, Brazil

    Naydja Moralles Maimone

  4. Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, São Paulo, Brazil

    Tiago F. Leão

  5. Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil

    Anelize Bauermeister

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    Taícia Pacheco Fill

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Gomes, P.W.P., de Tralia Medeiros, T.C., Maimone, N.M., Leão, T.F., de Moraes, L.A.B., Bauermeister, A. (2023). Microbial Metabolites Annotation by Mass Spectrometry-Based Metabolomics. In: Pacheco Fill, T. (eds) Microbial Natural Products Chemistry. Advances in Experimental Medicine and Biology(), vol 1439. Springer, Cham. https://doi.org/10.1007/978-3-031-41741-2_9

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