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Gold Nanoparticles-Based Point-of-Care Colorimetric Diagnostic for Plant Diseases

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Biosensors in Agriculture: Recent Trends and Future Perspectives

Part of the book series: Concepts and Strategies in Plant Sciences ((CSPS))

Abstract

Plant diseases caused by pathogens such as fungi, viruses, and bacteria create a substantial loss worldwide that leads to a severe threat to food security and human health. Therefore, disease management is a priority in agricultural-based countries. The detection of plant-pathogens is the primary step in disease management, which is crucial for plant health monitoring. Presently, few immunological methods are utilized to detect plant pathogens such as direct tissue blot immunoassay, enzyme-linked immunosorbent assay, and fluorometric immunoassay. DNA-based detection methods are also keenly applied for plant pathogen identification and detection, such as polymerase chain reaction, real-time PCR, and dot blot hybridization. However, these detection methods are inherited with some disadvantages like costly antibodies, complicated procedures, challenge to produce specific antibodies, expensive equipment, and susceptibility to contamination. This chapter reviews an instrument-free, instant, highly selective, and sensitive colorimetric detection of plant pathogens. The colorimetric detection is working on localized surface plasmon resonance properties of AuNPs, which causes the colour change of assay.

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Authors and Affiliations

  1. Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Sector 125, Noida, UP, 201303, India

    Ravi Mani Tripathi

  2. Department of Pediatrics, Division of Pediatric Hematology, Oncology & Bone Marrow Transplant, University of Wisconsin–Madison, Madison, WI, 53706, USA

    Prashant Sharma

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  1. Ravi Mani Tripathi
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Correspondence to Ravi Mani Tripathi .

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Editors and Affiliations

  1. Amity Institute of Nanotechnology, Amity University, Noida, Uttar Pradesh, India

    Ramesh Namdeo Pudake

  2. Amity Institute of Nanotechnology, Amity University, Noida, Uttar Pradesh, India

    Utkarsh Jain

  3. Department of Atomic Energy, ICAR - National Institute for Plant Biotechnology, New Delhi, Delhi, India

    Chittaranjan Kole

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Tripathi, R.M., Sharma, P. (2021). Gold Nanoparticles-Based Point-of-Care Colorimetric Diagnostic for Plant Diseases. In: Pudake, R.N., Jain, U., Kole, C. (eds) Biosensors in Agriculture: Recent Trends and Future Perspectives. Concepts and Strategies in Plant Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-66165-6_10

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