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DNA Methylation in Mouse Testes

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PIWI-Interacting RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1093))

Abstract

DNA methylation of retrotransposons and imprinted genes is accurately regulated in spermatogenesis. In particular, CpG methylation of long interspersed elements-1 (LINE1 or L1) and intracisternal A-particle (IAP) retrotransposons during spermatogenesis has been well characterized. CpG methylation of the regulatory regions of retrotransposons is acquired during embryonic testis development; however, reductions of DNA methylation in LINE1 and/or IAP and/or Rasgrf1, which is an imprinted gene, are observed in deficient mice of piRNA biogenesis concerning. Here, we describe two methods, bisulfite sequencing and Southern blotting using a methylation-sensitive restriction enzyme, for analysis of DNA methylation of LINE1, IAP, and imprinted genes in mouse testes.

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Acknowledgments

The author thanks Ms. N. Asada for technical support. This work was supported in part by grants from the Japan Society for the Promotion of Science, the Ministry of Education, Science, Sports, and Culture.

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Authors and Affiliations

  1. Department of Pathology, Medical School, Osaka University, Osaka, Japan

    Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima & Toru Nakano

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  1. Satomi Kuramochi-Miyagawa
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  2. Kanako Kita-Kojima
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  3. Yusuke Shiromoto
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  4. Daisuke Ito
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  5. Hirotaka Koshima
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  6. Toru Nakano
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Editors and Affiliations

  1. The University of Tokyo Dept of Biophysics & Biochemistry, Tokyo, Japan

    Mikiko C. Siomi

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© 2014 Springer Science+Business Media, LLC

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Kuramochi-Miyagawa, S., Kita-Kojima, K., Shiromoto, Y., Ito, D., Koshima, H., Nakano, T. (2014). DNA Methylation in Mouse Testes. In: Siomi, M. (eds) PIWI-Interacting RNAs. Methods in Molecular Biology, vol 1093. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-694-8_8

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  • DOI: https://doi.org/10.1007/978-1-62703-694-8_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-693-1

  • Online ISBN: 978-1-62703-694-8

  • eBook Packages: Springer Protocols

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