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PIWI-Interacting RNAs

Methods and Protocols

  • Mikiko C. Siomi

Part of the Methods in Molecular Biology book series (MIMB, volume 1093)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Yuzo Niki, Takuya Sato, Takafumi Yamaguchi, Ayaka Saisho, Hiroshi Uetake, Hidenori Watanabe
    Pages 13-23
  3. Shinpei Kawaoka, Susumu Katsuma, Yukihide Tomari
    Pages 35-46
  4. Ivan Olovnikov, Adrien Le Thomas, Alexei A. Aravin
    Pages 47-58
  5. Takeo Suzuki, Kenjyo Miyauchi, Yuriko Sakaguchi, Tsutomu Suzuki
    Pages 59-72
  6. Anastassios Vourekas, Zissimos Mourelatos
    Pages 73-95
  7. Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, Toru Nakano
    Pages 97-109
  8. Jessica A. Matts, Yuliya Sytnikova, Gung-wei Chirn, Gabor L. Igloi, Nelson C. Lau
    Pages 123-136
  9. Shozo Honda, Yoriko Kirino, Yohei Kirino
    Pages 137-148
  10. Sergey Shpiz, Sergey Lavrov, Alla Kalmykova
    Pages 161-169
  11. Thomas Grentzinger, Séverine Chambeyron
    Pages 171-182
  12. Hsin-Yi Huang, René F. Ketting
    Pages 183-194
  13. Jon McGinn, Benjamin Czech
    Pages 195-208
  14. Tomoko Noto, Henriette M. Kurth, Kazufumi Mochizuki
    Pages 209-224
  15. Tomoko Noto, Henriette M. Kurth, Kazufumi Mochizuki
    Pages E1-E2
  16. Back Matter
    Pages 247-249

About this book

Introduction

PIWI-interacting RNAs (piRNAs) are the third and most-recently discovered group of silencing-inducible small RNAs in animals. PIWI-Interacting RNAs: Methods and Protocols provides the most recent methods and protocols for studying piRNAs in the gonads of a wide range of species, as well as in any other organs where piRNAs may be detected. Comprehensive high-throughput sequencing analysis of piRNAs in embryos, testes and ovaries of D. melanogaster, as well as in mouse and rat testes, has raised the profile of piRNAs and thus further accelerated piRNA studies. In addition to C. elegans, other model species such as Drosophila melanogaster, Arabidopsis thaliana and mice, along with cultured cell lines such as HeLa and Drosophila Schneider 2 (S2), and other such organisms have been used to address the fundamental questions of the biogenesis and functions of RNAi-triggering small non-coding RNAs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PIWI-Interacting RNAs: Methods and Protocols seeks to serve both established researchers and newcomers to the field to progress towards the ultimate goal of understanding the mechanisms and actions of piRNAs.

Keywords

PIWI proteins PIWI-interacting RNAs PiRNAs RNAi non-coding RNAs

Editors and affiliations

  • Mikiko C. Siomi
    • 1
  1. 1.The University of Tokyo Dept of Biophysics & BiochemistryTokyoJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-694-8
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-693-1
  • Online ISBN 978-1-62703-694-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site