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N-Glycoprotein Enrichment by Lectin Affinity Chromatography

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Plant Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1072))

Abstract

Lectins are proteins that bind to sugars with varying specificities and several have been identified that show differential binding to structurally variable glycans attached to glycoproteins. Consequently, lectin affinity chromatography represents a valuable tool for glycoproteome studies, allowing enrichment of glycoproteins in samples prior to their identification by mass spectrometry (MS). From the perspective of plant scientists, lectin enrichment has proven useful for studies of the proteomes of the secretory pathways and cell wall, due to the high proportion of constituent proteins that are glycosylated. This chapter outlines a strategy to generate samples enriched with glycoproteins from bulk plant tissues prior to further characterization by MS, or other techniques.

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Acknowledgments

Funding to JKCR for research in this area is provided by the NSF Plant Genome Research Program (DBI-0606595) and the New York State Office of Science, Technology and Academic Research (NYSTAR).

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Authors and Affiliations

  1. Department of Plant Biology, Cornell University, Ithaca, NY, USA

    Eliel Ruiz-May & Jocelyn K. C. Rose

  2. Boyce Thompson Institute for Plant Research, Department of Plant Biology, Cornell University, Ithaca, NY, USA

    Carmen Catalá

Authors
  1. Eliel Ruiz-May
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  2. Carmen Catalá
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  3. Jocelyn K. C. Rose
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Editor information

Editors and Affiliations

  1. Dept of Biochem & Molecular Bio, University of Cordoba, Cordoba, Spain

    Jesus V. Jorrin-Novo

  2. University of Tsukuba National Institute of Crop Science, Tsukuba, Japan

    Setsuko Komatsu

  3. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Wolfram Weckwerth

  4. Dept. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Stefanie Wienkoop

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Ruiz-May, E., Catalá, C., Rose, J.K.C. (2014). N-Glycoprotein Enrichment by Lectin Affinity Chromatography. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_43

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  • DOI: https://doi.org/10.1007/978-1-62703-631-3_43

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-630-6

  • Online ISBN: 978-1-62703-631-3

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