Abstract
We are currently using a 2-DE-based proteomics approach to study plant responses to pathogenic fungi by using the carnation (Dianthus caryophyllus L)–Fusarium oxysporum f. sp. dianthi pathosystem. It is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research. The optimization procedure for the extraction and separation of proteins by 1-DE and 2-DE in the indicated system is reported. This strategy can be extrapolated to other plant–pathogen interaction systems in order to perform an evaluation of the changes in the host protein profile caused by the pathogen and to identify proteins which, at early stages, are involved or implicated in the plant defense response.
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Acknowledgments
This work was supported by the University of Córdoba (AGR-0164: Agroforestry and Plant Biochemistry and Proteomics Research Group), the Regional Government of Andalusia (Junta de Andalucía), Universidad Nacional de Colombia (Bogotá), and COLCIENCIAS (Colombia).
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Ardila, H.D., Fernández, R.G., Higuera, B.L., Redondo, I., Martínez, S.T. (2014). Protein Extraction and Gel-Based Separation Methods to Analyze Responses to Pathogens in Carnation (Dianthus caryophyllus L). In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_39
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DOI: https://doi.org/10.1007/978-1-62703-631-3_39
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