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Spatiotemporal Visualization of DNA Replication Dynamics

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Imaging Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1042))

Abstract

The ability of cells to copy their DNA allows them to transmit their genetic information to their progeny. In such, this central biological process preserves the instructions that direct the entire development of a cell. Earlier biochemical analysis in vitro and genetic analysis in yeast laid the basis of our understanding of the highly conserved mechanism of DNA replication. Recent advances on labeling and live-cell microscopy permit now the dissection of this fundamental process in vivo within the context of intact cells. In this chapter, we describe in detail how to perform multiple DNA replication labeling and detection allowing high spatial resolution imaging, as well as how to follow DNA replication in living cells allowing high temporal resolution imaging.

Marius Reinhart and Corella S. Casas-Delucchi have contributed equally to this work.

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Acknowledgements

We thank Juan Alberto Marchal (University of Jaen, Spain) for the Microtus cabrerae fibroblasts and all present and past members of the laboratory for their contributions over the years. The laboratory of M. Cristina Cardoso is supported by grants of the German Research Foundation (DFG) and the Federal Ministry of Education and Research (BMBF).

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Authors and Affiliations

  1. Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany

    Marius Reinhart, Corella S. Casas-Delucchi & M. Cristina Cardoso

Authors
  1. Marius Reinhart
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  2. Corella S. Casas-Delucchi
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  3. M. Cristina Cardoso
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Editor information

Editors and Affiliations

  1. Bar-Ilan University Inst. of Nanotech & Advanced Materials, Ramat Gan, Israel

    Yaron Shav-Tal

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Reinhart, M., Casas-Delucchi, C.S., Cardoso, M.C. (2013). Spatiotemporal Visualization of DNA Replication Dynamics. In: Shav-Tal, Y. (eds) Imaging Gene Expression. Methods in Molecular Biology, vol 1042. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-526-2_15

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  • DOI: https://doi.org/10.1007/978-1-62703-526-2_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-525-5

  • Online ISBN: 978-1-62703-526-2

  • eBook Packages: Springer Protocols

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