Abstract
Cytokine production by activated microglia is one of the hallmarks of inflammatory response in the CNS. The cytokines released by microglia cells can be very different depending on the proinflammatory stimulus.
Traditionally, to quantify these different cytokines, the “Sandwich”-enzyme-linked immunosorbent assay (Sandwich-ELISA) has been used. In this chapter we will discuss and describe an improved protocol of the Sandwich-ELISA developed by Meso-Scale Discovery based on an electrochemiluminescence detection system, which allows the ultralow detection of multiple cytokines in microglia cell supernatant.
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Acknowledgement
This work has been supported by a grant provided by the Swedish Research Council.
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Burguillos, M.A. (2013). Use of Meso-Scale Discovery™ to Examine Cytokine Content in Microglia Cell Supernatant. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_11
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DOI: https://doi.org/10.1007/978-1-62703-520-0_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-519-4
Online ISBN: 978-1-62703-520-0
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