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Sampling and Analysis of Phloem Sap

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Plant Mineral Nutrients

Part of the book series: Methods in Molecular Biology ((MIMB,volume 953))

Abstract

The transport tubes of the phloem are essential for higher plants. They not only provide the route for the distribution of assimilates produced during photosynthesis from source to sink organs but also (re-) distribute mineral nutrients. Additionally, the phloem is essential for sending information between distant plant organs and steering developmental and defense processes. For example, flowering and tuberization time are controlled by phloem-mobile signals and important defense reactions on the whole plant level, like systemic acquired resistance or systemic gene silencing, are spread through the phloem. In addition, recent results demonstrate that also the allocation of mineral nutrients is coordinated by phloem mobile signaling molecules.

However, in many studies the important analysis of phloem sap is neglected, probably because the content of sieve tubes is not easy to access. This chapter will describe the current methods for sampling and analysis of phloem sap in order to encourage researchers to include the analysis of this crucial compartment in their relevant studies.

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Author information

Authors and Affiliations

  1. Institut Jean Pierre Bourgin (IJPB), INRA-AgroParisTech, Versailles, France

    Sylvie Dinant

  2. Centro de Biotecnología y Genómica de Plantas (CBGP), Campus de Montegancedo, Madrid, Spain

    Julia Kehr

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  1. Sylvie Dinant
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  2. Julia Kehr
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Correspondence to Julia Kehr .

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Editors and Affiliations

  1. University of York, Biology Department Area 9, York, YO10 5DD, United Kingdom

    Frans J.M. Maathuis

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Dinant, S., Kehr, J. (2013). Sampling and Analysis of Phloem Sap. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_12

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  • DOI: https://doi.org/10.1007/978-1-62703-152-3_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-151-6

  • Online ISBN: 978-1-62703-152-3

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