Abstract
This volume of “Methods in Molecular Biology” entitled “Phytoplasmas: Methods and Protocols” aims to provide a broad range of protocols for working with this group of plant pathogens. In this first chapter, we provide some background information about the phytoplasmas to put the protocols into context.
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References
Doi Y et al (1967) Mycoplasma—or PLT group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches’ broom, aster yellows, or paulownia witches’ broom. Ann Phytopathol Soc Jpn 33:259–266
Lee I-M, Davis RE, Gundersen-Rindal DE (2000) Phytoplasmas: phytopathogenic mollicutes. Annu Rev Microbiol 56:1593–1597
Firrao G, Gibb K, Streten C (2005) Short taxonomic guide to the genus ‘Candidatus phytoplasma’. J Plant Pathol 87:249–263
Lee I-M et al (1998) Revised classification scheme of phytoplasmas based an RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169
Wei W et al (2007) Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Evol Microbiol 57:1855–1867
Wei W et al (2008) Automated RFLP pattern comparison and similarity coefficient calculation for rapid delineation of new and distinct phytoplasma 16Sr subgroup lineages. Int J Syst Evol Microbiol 58:2368–2377
Nei M, Li W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. PNAS USA 76:5269–5273
Christensen NM et al (2004) Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging. Mol Plant Microbe Interact 17:1175–1184
Choi YH et al (2004) Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1H-NMR spectroscopy and multivariate data analysis. Plant Physiol 135:2398–2410
Pracos P et al (2006) Tomato flower abnormalities induced by stolbur phytoplasma infection are associated with changes of expression of floral development genes. Mol Plant Microbe Interact 19:62–68
Oshima K et al (2004) Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma. Nat Genet 36:27–29
Bai X et al (2006) Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts. J Bacteriol 188:3682–3696
Tran-Nguyen LTT et al (2008) Comparative genome analysis of ‘Candidatus Phytoplasma australiense’ (subgroup tuf Australia I; rp-A) and ‘Ca. Phytoplasma asteris’ strains OY-M and AY-WB. J Bacteriol 190:3979–3991
Kube M et al (2008) The linear chromosome of the plant-pathogenic mycoplasma ‘Candidatus Phytoplasma mali’. BMC Genomics 9:306
Christensen NM et al (2005) Phytoplasmas and their interactions with hosts. Trends Plant Sci 10:526–535
Oshima K, Nishida H (2007) Phylogenetic relationships among mycoplasmas based on the whole genomic information. J Mol Evol 65:249–258
Dickinson M (2010) Mobile units of DNA in phytoplasma genomes. Mol Microbiol 77:1351–1353
Toruño TY et al (2010) Phytoplasma PMU1 exists as linear chromosomal and circular extrachromosomal elements and has enhanced expression in insect vectors compared with plant hosts. Mol Microbiol 77:1406–1415
Hoshi A et al (2008) A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium. PNAS USA 106:6416–6421
Bai X et al (2009) AY-WB phytoplasma secretes a protein that targets plant cell nuclei. Mol Plant Microbe Interact 22:18–30
Himeno M et al (2011) Unique morphological changes in plant pathogenic phytoplasma-infected petunia flowers are related to transcriptional regulation of floral homeotic genes in an organ-specific manner. Plant J 67:971–979
MacLean AM et al (2011) Phytoplasma effector SAP54 induces indeterminate leaf-like flower development in Arabidopsis plants. Plant Physiol 157:831–841
Weintraub PG, Beanland Le A (2006) Insect vectors of phytoplasmas. Annu Rev Entomol 51:91–111
Lefol C et al (1994) Propagation of flavescence doree MLO (Mycoplasma-Like Organism) in the leafhopper vector Euscelidius variegatus. J Invertebr Pathol 63:285–293
Hogenhout SA et al (2008) Phytoplasmas: bacteria that manipulate plants and insects. Mol Plant Pathol 9:403–423
Bressan A, Girolami V, Boudon-Padieu E (2005) Reduced fitness of the leafhopper vector Scaphoideus titanus exposed to Flavescence dorée phytoplasma. Entomol Exp Appl 115:283–290
Beanland L et al (2000) Influence of aster yellows phytoplasma on the fitness of aster leafhopper (Homoptera: Cicadellidae). Ann Entomol Soc Am 93:271–276
Cordova I et al (2003) In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease. Mol Plant Pathol 4:99–108
Nipah JO, Jones P, Dickinson MJ (2007) The Cape St Paul Wilt disease is transmitted to the embryos of infected West African tall coconut palms. Plant Pathol 56:777–784
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Dickinson, M., Tuffen, M., Hodgetts, J. (2013). The Phytoplasmas: An Introduction. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_1
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DOI: https://doi.org/10.1007/978-1-62703-089-2_1
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-089-2
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