Abstract
Production of gametocytes in vitro is essential for studies of Plasmodium falciparum sexual stages. Here, we describe procedures for the high-yield production and fractionation of P. falciparum gametocytes stages I to V.
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Acknowledgments
We thank Kim Williamson for helpful advice and discussions about production of healthy gametocytes. We also thank Jun Cao for help with Percoll-sorbitol purification protocols and advice on synchronization.
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Saliba, K.S., Jacobs-Lorena, M. (2012). Production of Plasmodium falciparum Gametocytes In Vitro. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_2
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DOI: https://doi.org/10.1007/978-1-62703-026-7_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-025-0
Online ISBN: 978-1-62703-026-7
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