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Production of Haploids and Doubled Haploids in Maize

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 877))

Abstract

The in vivo haploid induction approach offers several advantages compared to the in vitro induction approach and recurrent self-pollination. It is currently the method of choice for inbred line development in many commercial maize breeding programs. Here, we describe the in vivo approach for generation of maternal doubled haploids (DHs). It involves four steps: (1) induction of haploidy by pollinating source germplasm with pollen of a haploid inducer; (2) identification of putative haploid seeds (seeds with a haploid embryo) using a seed coloration marker system; (3) doubling of chromosomes of putative haploids by treating seedlings with a mitotic inhibitor; and (4) verification of putative doubled haploids with a stalk color marker and self-pollination of true doubled haploid plants to multiply their seed.

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Acknowledgments

The scientific and editorial contributions of B. S. Dhillon, W. Schipprack, B. Devezi Savula, and H. H. Geiger are gratefully acknowledged. Vanessa Prigge is indebted to the Eiselen Foundation Ulm, Germany, for financial support of her work as a joint PhD student between CIMMYT and the University of Hohenheim, Germany.

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Authors and Affiliations

  1. Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, Stuttgart, Germany

    Vanessa Prigge & Albrecht E. Melchinger

Authors
  1. Vanessa Prigge
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  2. Albrecht E. Melchinger
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Corresponding author

Correspondence to Albrecht E. Melchinger .

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Editors and Affiliations

  1. , Unidad de Bioquímica y Biología Molecula, Centro de Investigación Científica de Yu, Apartado Postal 87, Cordemex, Mérida, Mexico

    Víctor M. Loyola-Vargas

  2. , Departamento de Ingeniería Genética de P, Ctr Investigación, Estudios Avanzados In, Apartado Postal 629, Irapuato, 36821, Mexico

    Neftalí Ochoa-Alejo

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© 2012 Springer Science+Business Media, LLC

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Prigge, V., Melchinger, A.E. (2012). Production of Haploids and Doubled Haploids in Maize. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_13

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  • DOI: https://doi.org/10.1007/978-1-61779-818-4_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-817-7

  • Online ISBN: 978-1-61779-818-4

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