Plant Cell Culture Protocols

  • Víctor M. Loyola-Vargas
  • Neftalí Ochoa-Alejo

Part of the Methods in Molecular Biology book series (MIMB, volume 877)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo
    Pages 1-8
  3. Trevor Thorpe
    Pages 9-27
  4. Rosa M. Galáz-Ávalos, Sagrario Aguilar-Díaz, Pedro A. Xool-González, Silvia M. Huchín-May, Víctor M. Loyola-Vargas
    Pages 29-40
  5. Gregorio Godoy-Hernández, Felipe A. Vázquez-Flota
    Pages 41-48
  6. Lizbeth A. Castro-Concha, Rosa María Escobedo, María de Lourdes Miranda-Ham
    Pages 49-56
  7. Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, Qiaochun Wang
    Pages 81-93
  8. Michael E. Compton
    Pages 109-127
  9. Yuri Peña-Ramírez, Juan Juárez-Gómez, José Antonio González-Rodríguez, Manuel L. Robert
    Pages 129-141
  10. Yıldız Aka Kaçar, Ben Faber
    Pages 143-151
  11. Miguel Ángel Herrera-Alamillo, Manuel L. Robert
    Pages 153-160
  12. Vanessa Prigge, Albrecht E. Melchinger
    Pages 161-172
  13. Verónica Garrocho-Villegas, María Teresa de Jesús-Olivera, Estela Sánchez Quintanar
    Pages 173-182
  14. Neftalí Ochoa-Alejo
    Pages 227-231
  15. Juntaro Kato, Masahiro Mii
    Pages 233-245
  16. José Luis Couselo, Elena Corredoira, Ana M. Vieitez, Antonio Ballester
    Pages 247-263

About this book

Introduction

Cell culture methodologies have become standard procedures in most plant laboratories. Currently,  facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Keywords

clonal propagation culture induction epigenetic analysis haploid production organelle transformation plant biotechnology somatic embryogenesis

Editors and affiliations

  • Víctor M. Loyola-Vargas
    • 1
  • Neftalí Ochoa-Alejo
    • 2
  1. 1., Unidad de Bioquímica y Biología MoleculaCentro de Investigación Científica de YuCordemex, MéridaMexico
  2. 2., Departamento de Ingeniería Genética de PCtr Investigación, Estudios Avanzados InIrapuatoMexico

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-818-4
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-817-7
  • Online ISBN 978-1-61779-818-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book