Abstract
Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semi-permeable membrane barrier.
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Acknowledgments
L. Facci was supported by Fondazione CARIPARO Progetto Dottorati di Ricerca’ Anno 2009.
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Skaper, S.D., Facci, L. (2012). Central Nervous System Neuron-Glia Co-culture Models. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 846. Humana Press. https://doi.org/10.1007/978-1-61779-536-7_8
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DOI: https://doi.org/10.1007/978-1-61779-536-7_8
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Online ISBN: 978-1-61779-536-7
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