Abstract
Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical and hippocampal neuron cell cultures, using either embryonic or postnatal tissue.
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Acknowledgments
L. Facci was supported by Fondazione CARIPARO Progetto Dottorati di Ricerca’ Anno 2009.
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Facci, L., Skaper, S.D. (2012). Amyloid β-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 846. Humana Press. https://doi.org/10.1007/978-1-61779-536-7_6
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DOI: https://doi.org/10.1007/978-1-61779-536-7_6
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