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Generation of Human Osteoclasts from Peripheral Blood

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.

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Author information

Authors and Affiliations

  1. Nordic Bioscience A/S, Herlev, Denmark

    Kim Henriksen & Morten A. Karsdal

  2. Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK

    Adam Taylor & Fraser P. Coxon

  3. Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK

    Denise Tosh

Authors
  1. Kim Henriksen
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  2. Morten A. Karsdal
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  3. Adam Taylor
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  4. Denise Tosh
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  5. Fraser P. Coxon
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Corresponding author

Correspondence to Kim Henriksen .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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Henriksen, K., Karsdal, M.A., Taylor, A., Tosh, D., Coxon, F.P. (2012). Generation of Human Osteoclasts from Peripheral Blood. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_11

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

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