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Rapid Selection of High-Affinity Binders Using Ribosome Display

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Ribosome Display and Related Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 805))

Abstract

Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail.

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Acknowledgments

We thank many former and current members of the Plückthun laboratory, mentioned in the references, for establishing and continuously optimizing the ribosome display protocol.

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Authors and Affiliations

  1. Department of Biochemistry, University of Zurich, Zurich, Switzerland

    Birgit Dreier & Andreas Plückthun

Authors
  1. Birgit Dreier
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  2. Andreas Plückthun
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Corresponding author

Correspondence to Andreas Plückthun .

Editor information

Editors and Affiliations

  1. , Antibody Discovery & Protein Engineering, MedImmune Limited, Granta Park, Cambridge, CB21 6GH, United Kingdom

    Julie A. Douthwaite

  2. , Antibody Discovery & Protein Engineering, MedImmune Limited, Granta Park, Cambridge, CB21 6GH, United Kingdom

    Ronald H. Jackson

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Support

Work on ribosome display was supported by the Swiss National Science Foundation.

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Dreier, B., Plückthun, A. (2012). Rapid Selection of High-Affinity Binders Using Ribosome Display. In: Douthwaite, J., Jackson, R. (eds) Ribosome Display and Related Technologies. Methods in Molecular Biology, vol 805. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-379-0_15

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  • DOI: https://doi.org/10.1007/978-1-61779-379-0_15

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-378-3

  • Online ISBN: 978-1-61779-379-0

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