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Ribosome Display and Related Technologies

Methods and Protocols

  • Julie A. Douthwaite
  • Ronald H. Jackson

Part of the Methods in Molecular Biology book series (MIMB, volume 805)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Review

    1. Front Matter
      Pages 1-1
    2. Andreas Plückthun
      Pages 3-28
  3. Translation Extract Preparation

  4. Basic Ribosome Display and Related Selection Methods

    1. Front Matter
      Pages 43-43
    2. Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, Yoshihiro Ito
      Pages 59-73
    3. Mingyue He, Bryan M. Edwards, Damjana Kastelic, Michael J. Taussig
      Pages 75-85
    4. Rong Wang, Steve W. Cotten, Rihe Liu
      Pages 87-100
    5. Miriam Kaltenbach, Florian Hollfelder
      Pages 101-111
    6. Shingo Ueno, Naoto Nemoto
      Pages 113-135
  5. Applications of Ribosome Display Methods Using Natural Amino Acids

    1. Front Matter
      Pages 137-137
    2. Yuki Mochizuki, Naoto Nemoto
      Pages 237-250
    3. Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, Takuya Ueda
      Pages 251-259
    4. Birgit Dreier, Andreas Plückthun
      Pages 261-286
    5. Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, Rihe Liu
      Pages 287-297
    6. Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-Laurance, Stéphane Colinet, Frédéric Pecorari
      Pages 315-331
  6. Incorporation of Non-natural Amino Acids for Selection by Ribosome Display and Related Methods

    1. Front Matter
      Pages 333-333
    2. Patrick C. Reid, Yuki Goto, Takayuki Katoh, Hiroaki Suga
      Pages 335-348
    3. R. Edward Watts, Anthony C. Forster
      Pages 349-365
  7. Case Studies

  8. Back Matter
    Pages 423-424

About this book

Introduction

Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread.  Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols.  The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.

Keywords

High affinity antibodies Natural amino acids Non-natural amino acids Peptides Protein engineering Selection methods Transcription cDNA mRNA display

Editors and affiliations

  • Julie A. Douthwaite
    • 1
  • Ronald H. Jackson
    • 2
  1. 1., Antibody Discovery & Protein EngineeringMedImmune LimitedCambridgeUnited Kingdom
  2. 2., Antibody Discovery & Protein EngineeringMedImmune LimitedCambridgeUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-379-0
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-378-3
  • Online ISBN 978-1-61779-379-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site