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Inkjet Printing for the Production of Protein Microarrays

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Protein Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 785))

Abstract

A significant proportion of protein microarray researchers would like the arrays they develop to become widely used research, screening, validation or diagnostic devices. For this to be achievable the arrays must be compatible with high-throughput techniques that allow manufacturing scale production. In order to simplify the transition from laboratory bench to market, Arrayjet have developed a range of inkjet microarray printers, which, at one end of the scale, are suitable for R&D and, at the other end, are capable of true high-throughput array output. To maintain scalability, all Arrayjet microarray printers utilise identical core technology comprising a JetSpyder™ liquid handling adaptor, which enables automated loading of an industry standard inkjet printhead compatible with non-contact on-the-fly printing. This chapter contains a detailed explanation of Arrayjet technology followed by a historical look at the development of inkjet technologies for protein microarray production. The method described subsequently is a simple example of an antibody array printed onto nitrocellulose-coated slides with specific detection with fluorescently labelled IgG. The method is linked to a notes section with advice on best practice and sources of useful information for protein microarray production using inkjet technology.

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Author information

Authors and Affiliations

  1. Arrayjet Ltd., MIC, Roslin, UK

    Iain McWilliam, Marisa Chong Kwan & Duncan Hall

Authors
  1. Iain McWilliam
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  2. Marisa Chong Kwan
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  3. Duncan Hall
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Corresponding author

Correspondence to Iain McWilliam .

Editor information

Editors and Affiliations

  1. (DKFZ), Division of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, Heidelberg, 69120, Germany

    Ulrike Korf

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McWilliam, I., Kwan, M.C., Hall, D. (2011). Inkjet Printing for the Production of Protein Microarrays. In: Korf, U. (eds) Protein Microarrays. Methods in Molecular Biology, vol 785. Humana Press. https://doi.org/10.1007/978-1-61779-286-1_23

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  • DOI: https://doi.org/10.1007/978-1-61779-286-1_23

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-285-4

  • Online ISBN: 978-1-61779-286-1

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