Abstract
The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells’ invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.
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References
Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. (2003) Cell migration: integrating signals from front to back. Science 302:1704–9
Kassis J, Lauffenburger DA, Turner T, Wells A. (2001) Tumor invasion as dysregulated cell motility. Semin Cancer Biol. 11:105–17
Sala G, Dituri F, Raimondi C, Previdi S, Maffucci T, Mazzoletti M, Rossi C, Iezzi M, Lattanzio R, Piantelli M, Iacobelli S, Broggini M, Falasca M. (2008) Phospholipase Cgamma1 is required for metastasis development and progression. Cancer Res. 68:10187–96.
Howard J (2009) Mechanical signaling in networks of motor and cytoskeletal proteins. Annu Rev Biophys. 38:217–34.
Maffucci T, Raimondi C, Abu-Hayyeh S, Dominguez V, Sala G, Zachary I, Falasca M. (2009) A phosphoinositide 3-kinase/phospholipase Cgamma1 pathway regulates fibroblast growth factor-induced capillary tube formation. PLoS One. 4:e8285.
Albini A, Iwamoto Y, Kleinman HK, Martin GR, Aaronson SA, Kozlowski JM, McEwan RN. (1987) A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 47:3239–45.
Albini A, Benelli R. (2007) The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation. Nat Protoc. 2:504–11.
Maffucci T, Piccolo E, Cumashi A, Iezzi M, Riley AM, Saiardi A, Godage HY, Rossi C, Broggini M, Iacobelli S, Potter BV, Innocenti P, Falasca M. (2005) Inhibition of the phosphatidylinositol 3-kinase/Akt pathway by inositol pentakisphosphate results in antiangiogenic and antitumor effects. Cancer Res. 65:8339–49.
Maffucci T, Cooke FT, Foster FM, Traer CJ, Fry MJ, Falasca M. (2005) Class II phosphoinositide 3-kinase defines a novel signaling pathway in cell migration. J Cell Biol. 169:789–99.
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Falasca, M., Raimondi, C., Maffucci, T. (2011). Boyden Chamber. In: Wells, C., Parsons, M. (eds) Cell Migration. Methods in Molecular Biology, vol 769. Humana Press. https://doi.org/10.1007/978-1-61779-207-6_7
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DOI: https://doi.org/10.1007/978-1-61779-207-6_7
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