Cell Migration

Developmental Methods and Protocols

  • Claire M. Wells
  • Maddy Parsons

Part of the Methods in Molecular Biology book series (MIMB, volume 769)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Miguel Vicente-Manzanares, Alan Rick Horwitz
    Pages 1-24
  3. Giles Cory
    Pages 25-30
  4. Sally T. Fram, Claire M. Wells, Gareth E. Jones
    Pages 31-40
  5. Sanjay Chaubey, Anne J. Ridley, Claire M. Wells
    Pages 41-51
  6. Samantha J. King, Maddy Parsons
    Pages 53-64
  7. Marco Falasca, Claudio Raimondi, Tania Maffucci
    Pages 87-95
  8. John Marshall
    Pages 97-110
  9. Taylor W. Starnes, Christa L. Cortesio, Anna Huttenlocher
    Pages 111-136
  10. Beata Wojciak-Stothard
    Pages 137-147
  11. Robert J. Cain, Bárbara Borda d’Água, Anne J. Ridley
    Pages 167-190
  12. Shawn A. Galdeen, Alison J. North
    Pages 205-222
  13. Veronika Jenei, Maria L. Nystrom, Gareth J. Thomas
    Pages 223-232
  14. Ming-Ching Wong, Maria Martynovsky, Jean E. Schwarzbauer
    Pages 233-247
  15. Carolina G. A. Moreira, Jennifer C. Regan, Anna Zaidman-Rémy, Antonio Jacinto, Soren Prag
    Pages 249-260
  16. Philip M. Elks, Catherine A. Loynes, Stephen A. Renshaw
    Pages 261-275

About this book


Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study.  The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances.  Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D.  It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.


Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology.


3D imaging Biochemical assay systems Cellular movement Embryology Functional assay systems In vitro migration Signalling pathways

Editors and affiliations

  • Claire M. Wells
    • 1
  • Maddy Parsons
    • 2
  1. 1., Division of Cancer StudiesKing's College LondonLondonUnited Kingdom
  2. 2.Randall Division of Cell and, Molecular BiophysicsKing's College LondonLondonUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-207-6
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-206-9
  • Online ISBN 978-1-61779-207-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book