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Lectins as Tools to Select for Glycosylated Proteins

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Gel-Free Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 753))

Abstract

Glycosylation has been recognized as one of the most important modifications on proteins. The interactions between proteins and glycans are known to play an important role in many biological processes. Lectins are carbohydrate-binding proteins that can specifically interact with and select for carbohydrate structures. The technique of lectin affinity chromatography takes advantage of this specific interaction and enables the selection and purification of glycoproteins with carbohydrate structures complementary to the lectin-binding site. Depending on the carbohydrate specificity of the lectin glycoprotein fractions enriched for example, high mannose or complex N-glycans or O-glycans can be obtained. Afterward both the protein part and the glycan part can be analyzed in more detail allowing the identification of the interacting partners and the type of glycans involved.

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Acknowledgments

The financial support of the Research Council of Ghent University and Fund for Scientific Research-Flanders (G.0022.08) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Molecular Biotechnology, Ghent University, Ghent, Belgium

    Els J. M. Van Damme

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  1. Els J. M. Van Damme
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Correspondence to Els J. M. Van Damme .

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Editors and Affiliations

  1. , VIB Department of Medical Protein Resear, VIB and Ghent University, Albert Baertsoenkaai 3, Gent, 9000, Belgium

    Kris Gevaert

  2. , VIB Department of Medical Protein Resear, VIB and Ghent University, Albert Baertsoenkaai 3, Gent, 9000, Belgium

    Joël Vandekerckhove

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Van Damme, E.J.M. (2011). Lectins as Tools to Select for Glycosylated Proteins. In: Gevaert, K., Vandekerckhove, J. (eds) Gel-Free Proteomics. Methods in Molecular Biology, vol 753. Humana Press. https://doi.org/10.1007/978-1-61779-148-2_19

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  • DOI: https://doi.org/10.1007/978-1-61779-148-2_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-147-5

  • Online ISBN: 978-1-61779-148-2

  • eBook Packages: Springer Protocols

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