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Mass Spectrometry-Driven Proteomics: An Introduction

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Gel-Free Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 753))

Abstract

Proteins are reckoned to be the key actors in a living organism. By studying proteins, one engages into deciphering a complex series of events occurring during a protein’s life span. This starts at the creation of a protein, which is tightly controlled on both a transcriptional (Williams and Tyler, 2007, Curr Opin Genet Dev 17, 88–93) and a translational level (Van Der Kelen et al., 2009, Crit Rev Biochem Mol Biol 44, 143–168). During translation, a primary strand of amino acids undergoes a complex folding process in order to obtain a native three-dimensional protein structure (Gross et al., 2003, Cell 115, 739–750). Proteins take on a plethora of functions, such as complex formation, receptor activity, and signal transduction, which ultimately adds up to a cellular phenotype. Consequently, protein analysis is of major interest in molecular biology and involves annotating their presence and localization, as well as their modification state and biochemical context. To accomplish this, many methods have been developed over the last decades, and their general principles and important recent advances in large-scale protein analysis or proteomics are discussed in this review.

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Notes

  1. 1.

    UniProtKB/Swiss-Prot release 57.9.

  2. 2.

    The HUPO Proteomics Standards Initiative (PSI) defines community standards for data representation in proteomics to facilitate data comparison, exchange, and verification. http://www.psidev.info/

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Acknowledgment

K.H. is supported by a Ph.D. grant from the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen).

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Authors and Affiliations

  1. Department of Medical Protein Research, VIB, Ghent University, B-9000, Ghent, Belgium

    Kenny Helsens & Lennart Martens

  2. Department of Biochemistry, Ghent University, B-9000, Ghent, Belgium

    Kenny Helsens & Lennart Martens

  3. VIB Department of Medical Protein Research and UGent Department of Biochemistry, VIB and Ghent University, Ghent, 9000, Belgium

    Joël Vandekerckhove & Kris Gevaert

Authors
  1. Kenny Helsens
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  2. Lennart Martens
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  3. Joël Vandekerckhove
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  4. Kris Gevaert
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Corresponding author

Correspondence to Kenny Helsens .

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Editors and Affiliations

  1. , VIB Department of Medical Protein Resear, VIB and Ghent University, Albert Baertsoenkaai 3, Gent, 9000, Belgium

    Kris Gevaert

  2. , VIB Department of Medical Protein Resear, VIB and Ghent University, Albert Baertsoenkaai 3, Gent, 9000, Belgium

    Joël Vandekerckhove

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Helsens, K., Martens, L., Vandekerckhove, J., Gevaert, K. (2011). Mass Spectrometry-Driven Proteomics: An Introduction. In: Gevaert, K., Vandekerckhove, J. (eds) Gel-Free Proteomics. Methods in Molecular Biology, vol 753. Humana Press. https://doi.org/10.1007/978-1-61779-148-2_1

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  • DOI: https://doi.org/10.1007/978-1-61779-148-2_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-147-5

  • Online ISBN: 978-1-61779-148-2

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