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Phaseolus Immature Embryo Rescue Technology

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Plant Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 710))

Abstract

Predominant among the production constraints of the common bean Phaseolus vulgaris are infestation of Ascochyta blight, Bean Golden Mosaic virus (BGMV), and Bean Fly. Interbreeding with Phaseolus ­coccineus L. and/or Phaseolus polyanthus Greenm has been shown to provide P. vulgaris with greater resistance to these diseases. For interspecific crosses to be successful, it is important to use P. coccineus and P. polyanthus as female parents; this prevents rapid reversal to the recurrent parent P. vulgaris. Although incompatibility barriers are post-zygotic, early hybrid embryo abortion limits the success of F1 crosses. While rescue techniques for globular and early heart-shaped embryos have improved in recent years, ­success in hybridization remains very low. In this study, we describe six steps that allowed us to rescue 2-day-old P. vulgaris embryos using a pod culture technique. Our methods consisted of (i) pod culture, (ii) extraction and culture of immature embryos, (iii) dehydration of embryos, (iv) germination of embryos, (v) rooting of developed shoots, and (vi) hardening of plantlets.

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Author information

Authors and Affiliations

  1. Life Science Department, Walloon Agricultural Research Center, Gembloux, Belgium

    Pascal Geerts

  2. Unité de Phytotechnie tropicale et d’Horticulture, Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgium

    André Toussaint, Guy Mergeai & Jean-Pierre Baudoin

Authors
  1. Pascal Geerts
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  2. André Toussaint
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  3. Guy Mergeai
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  4. Jean-Pierre Baudoin
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Corresponding author

Correspondence to Pascal Geerts .

Editor information

Editors and Affiliations

  1. Dept. Biological Sciences, University of Calgary, University Dr. NW., 2500, Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

  2. , Department of Biological Sciences, University of Calgary, University Dr. NW 2500, Calgary, T2N 1N4, Alberta, Canada

    Edward C. Yeung

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© 2011 Humana Press

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Geerts, P., Toussaint, A., Mergeai, G., Baudoin, JP. (2011). Phaseolus Immature Embryo Rescue Technology. In: Thorpe, T., Yeung, E. (eds) Plant Embryo Culture. Methods in Molecular Biology, vol 710. Humana Press. https://doi.org/10.1007/978-1-61737-988-8_10

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  • DOI: https://doi.org/10.1007/978-1-61737-988-8_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-987-1

  • Online ISBN: 978-1-61737-988-8

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