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In Vivo Treg Suppression Assays

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Regulatory T Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 707))

Abstract

To fully examine the functionality of a regulatory T cell (Treg) population, one needs to assess their ability to suppress in a variety of in vivo models. We describe five in vivo models that examine the suppressive capacity of Tregs upon different target cell types. The advantages and disadvantages of each model including resources, time, and technical expertise required to execute each model are also described.

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Acknowledgments

We thank Terrence Geiger and Hongbo Chi for advice and critical discussion and Samir Burns for technical guidance regarding EAE experiments. We are also grateful to Mary Jo Turk for advice and technical guidance regarding the B16 tumor model. We thank Karen Forbes, Tara Moore, Jessica Magwood, and Amy Krause for maintenance and breeding of mouse colonies, Andrea Szymczak-Workman for IBD histological analysis, Richard Cross, Greig Lennon and Stephanie Morgan for FACS, the St Jude VPC Laboratory for histological analyses, the staff of the Shared Animal Resource Center at St Jude for the animal husbandry, Matthew Smeltzer for advice on statistical analysis and the Hartwell Center for Biotechnology and Bioinformatics at St Jude for MOG synthesis and purification. LWC is supported by an Individual NIH NRSA (F32 AI072816). MB is supported by a Juvenile Diabetes Research Foundation International postdoctoral fellowship (3-2009-594). DAAV is supported by the National Institutes of Health (NIH) (AI39480, AI52199, AI072239), Juvenile Diabetes Research Foundation International (1-2004-141 [The Robert and Janice Compton Research Grant, In Honor of Elizabeth S. Compton] and 1-2006-847), a Cancer Center Support CORE grant (CA21765) and the American Lebanese Syrian Associated Charities (ALSAC).

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Authors and Affiliations

  1. Department of Immunology, St. Jude Children’s Research Hospital, Memphis, TN, USA

    Creg J. Workman, Lauren W. Collison, Maria Bettini, Meenu R. Pillai & Dario A. A. Vignali

  2. Department of Pathology, St. Jude Children’s Research Hospital, Memphis, TN, USA

    Jerold E. Rehg

Authors
  1. Creg J. Workman
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  2. Lauren W. Collison
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  3. Maria Bettini
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  4. Meenu R. Pillai
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  5. Jerold E. Rehg
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  6. Dario A. A. Vignali
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Corresponding author

Correspondence to Dario A. A. Vignali .

Editor information

Editors and Affiliations

  1. , Division of Immunoregulation, MRC National Institute for Medical Resea, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom

    George Kassiotis

  2. , VIB Autoimmune Genetics Laboratory, Katholieke Universiteit Leuven, Herestraat 49, Leuven, 3000, Belgium

    Adrian Liston

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Workman, C.J., Collison, L.W., Bettini, M., Pillai, M.R., Rehg, J.E., Vignali, D.A.A. (2011). In Vivo Treg Suppression Assays. In: Kassiotis, G., Liston, A. (eds) Regulatory T Cells. Methods in Molecular Biology, vol 707. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-979-6_9

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  • DOI: https://doi.org/10.1007/978-1-61737-979-6_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-978-9

  • Online ISBN: 978-1-61737-979-6

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