Regulatory T Cells

Methods and Protocols

  • George Kassiotis
  • Adrian Liston

Part of the Methods in Molecular Biology book series (MIMB, volume 707)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Shimon Sakaguchi
      Pages 3-17
  3. In Vitro

    1. Front Matter
      Pages 19-19
    2. Lauren W. Collison, Dario A. A. Vignali
      Pages 21-37
    3. Nagendra Singh, Rafal Pacholczyk, Makio Iwashima, Leszek Ignatowicz
      Pages 39-44
    4. Ye Zheng
      Pages 71-82
    5. Milka Sarris, Alexander G. Betz
      Pages 83-101
  4. In Vivo

    1. Front Matter
      Pages 103-103
    2. Nadia M. Jeremiah, Adrian Liston
      Pages 105-118
    3. Creg J. Workman, Lauren W. Collison, Maria Bettini, Meenu R. Pillai, Jerold E. Rehg, Dario A. A. Vignali
      Pages 119-156
    4. Katharina Lahl, Tim Sparwasser
      Pages 157-172
    5. Carolin Daniel, Hidde Ploegh, Harald von Boehmer
      Pages 173-185
  5. Human

    1. Front Matter
      Pages 197-197
    2. Eva d’Hennezel, Ciriaco A. Piccirillo
      Pages 199-218
    3. Amy C. Hobeika, Michael A. Morse, Takuya Osada, Sharon Peplinski, H. Kim Lyerly, Timothy M. Clay
      Pages 219-231
    4. Milica Vukmanovic-Stejic, Yan Zhang, Arne N. Akbar, Derek C. Macallan
      Pages 243-261
    5. Barbara Fazekas de St Groth, Erhua Zhu, Suzanne Asad, Loretta Lee
      Pages 263-279
  6. Back Matter
    Pages 281-282

About this book


Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.


FoxP3+ Immune regulation Immune regulators In vitro and In vivo Lineage markers Suppressive mechanisms Transcription factors Tregs

Editors and affiliations

  • George Kassiotis
    • 1
  • Adrian Liston
    • 2
  1. 1., Division of ImmunoregulationMRC National Institute for Medical ReseaLondonUnited Kingdom
  2. 2., VIB Autoimmune Genetics LaboratoryKatholieke Universiteit LeuvenLeuvenBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61737-978-9
  • Online ISBN 978-1-61737-979-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site