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Knock-In Approaches

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Transgenic Mouse Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 693))

Abstract

Molecular genetic strategies to study gene function in mice or to generate a mouse model for a human disease are continuously under development. The application and importance of knock-in approaches are increasing. This chapter elaborates on novel developments for the generation of knock-in mice. Special emphasis is given to recombinase-mediated cassette exchange, a new emerging knock-in strategy that enables easy generation of a series of different knock-in mutations within one gene.

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Author information

Authors and Affiliations

  1. Laboratory for Experimental Mouse Genetics, Center for Human Genetics, K.U. Leuven, Leuven, Belgium

    Anton J. M. Roebroek, Philip L. S. M. Gordts & Sara Reekmans

Authors
  1. Anton J. M. Roebroek
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  2. Philip L. S. M. Gordts
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  3. Sara Reekmans
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Corresponding author

Correspondence to Anton J. M. Roebroek .

Editor information

Editors and Affiliations

  1. Dept. Pathology & Medical Biology, Medical Biology Section, University Medical Center Groningen, Antonius Deusinglaan 1, Groningen, 9713 AV, Netherlands

    Marten H. Hofker

  2. Mayo Clinic, First Street SW. 200, Rochester, 55905, Minnesota, USA

    Jan van Deursen

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© 2011 Springer Science+Business Media, LLC

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Roebroek, A.J.M., Gordts, P.L.S.M., Reekmans, S. (2011). Knock-In Approaches. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_15

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  • DOI: https://doi.org/10.1007/978-1-60761-974-1_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-973-4

  • Online ISBN: 978-1-60761-974-1

  • eBook Packages: Springer Protocols

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