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Quantification of Proteins by Label-Free LC-MS/MS

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LC-MS/MS in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 658))

Abstract

Quantitative proteomic profiling is becoming a widely used approach in systems biology and biomarker discovery. There is a growing realization that quantitative studies require high numbers of non-pooled samples for increased statistical power. We present a descriptive protocol for label-free quantitation of proteins by LC-MS/MS that enables to obtain both quantitative and qualitative information in one study without the need to pool samples or label them.

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Acknowledgment

The research was kindly supported by the Stanley Medical Research Institute (SMRI). We also thank Psynova Neurotech® for center support and for Ph.D. funding. The service team from Waters Corp. is acknowledged for its technical support and assistance.

We would also like to present our appreciation to Dr. Hassan Rahmoune and Mr. Emanuel Schwarz of the Cambridge Center for Neuropsychiatric Research for the helpful discussions and suggestions.

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Authors and Affiliations

  1. Institute of Biotechnology, University of Cambridge, Cambridge, UK

    Yishai Levin

  2. Institute of Biotechnology, University of Cambridge, Cambridge, UK

    Sabine Bahn

Authors
  1. Yishai Levin
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  2. Sabine Bahn
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Editor information

Editors and Affiliations

  1. Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, Charterhouse Square, London, EC1M 6BQ, United Kingdom

    Pedro R. Cutillas

  2. , EGA Institute for Women's Health, University College London, Gower Street, London, WC1E 6BT, United Kingdom

    John F. Timms

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Levin, Y., Bahn, S. (2010). Quantification of Proteins by Label-Free LC-MS/MS. In: Cutillas, P., Timms, J. (eds) LC-MS/MS in Proteomics. Methods in Molecular Biology, vol 658. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-780-8_13

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  • DOI: https://doi.org/10.1007/978-1-60761-780-8_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-779-2

  • Online ISBN: 978-1-60761-780-8

  • eBook Packages: Springer Protocols

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