LC-MS/MS in Proteomics

Methods and Applications

  • Pedro R. Cutillas
  • John F. Timms

Part of the Methods in Molecular Biology book series (MIMB, volume 658)

Table of contents

  1. Front Matter
    Pages i-x
  2. Overviews of Techniques and Approaches in LC-MS-Based Proteomics

    1. Front Matter
      Pages 1-1
    2. Robert Chalkley
      Pages 47-60
    3. Richard J. Jacob
      Pages 61-91
    4. Hannah Johnson, Claire E. Eyers
      Pages 93-108
  3. Structural Analysis of Post-Translational Modifications

  4. Techniques for Quantitative LC-MS

    1. Front Matter
      Pages 153-153
    2. Reiko Kiyonami, Bruno Domon
      Pages 155-166
    3. Andrew James, Claus Jorgensen
      Pages 167-185
    4. Kristoffer T.G. Rigbolt, Blagoy Blagoev
      Pages 187-204
    5. Richard D. Unwin
      Pages 205-215
    6. Yishai Levin, Sabine Bahn
      Pages 217-231
  5. Analysis of Protein Complexes and Organelles

    1. Front Matter
      Pages 233-233
    2. Andrew J. Thompson, Ritchie Williamson
      Pages 235-253
    3. Joern Dengjel, Lis Jakobsen, Jens S. Andersen
      Pages 255-265

About this book

Introduction

With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.

Keywords

Biological fluids and clinical samples Chromatography Liquid chromatography Mass Spectrometry Post-translational modifications Protein complexes and organelles Proteomics Quantitative LC-MS Structural analysis Tandem mass spectrometry Translation protein complexes proteins

Editors and affiliations

  • Pedro R. Cutillas
    • 1
  • John F. Timms
    • 2
  1. 1.Bart’s and the London School of Medicine, Institute of CancerQueen Mary, Universtiy of LondonLondonUnited Kingdom
  2. 2., EGA Institute for Women's HealthUniversity College LondonLondonUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-780-8
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-779-2
  • Online ISBN 978-1-60761-780-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book