Abstract
In most eukaryotes, meiotic crossovers (COs) are non-randomly placed along the bivalents, such that the presence of a CO reduces the probability of additional COs nearby. This phenomenon, named CO interference, was originally defined genetically, but can also be analyzed cytologically by studying the chromosomal positions of protein complexes that are involved in CO formation, or by studying the positions of chiasmata. Here we focus on the cytological analysis of interference among protein complexes involved in meiotic recombination and CO formation in the mouse. During the pachytene stage of meiosis, these protein complexes can be visualized as immunofluorescent foci along synaptonemal complexes (SCs), which are linear protein structures that are formed along homologous chromosome pairs (bivalents) during meiotic prophase. We describe how to make cytological preparations that are suitable for the analysis of interference among these foci, and how to estimate the strength of interference among foci, using the gamma distribution as a mathematical model for focus/CO positioning.
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References
Hillers, K. J. (2004) Crossover interference. Curr. Biol. 14, R1036–R1037.
de Boer, E., Stam, P., Dietrich, A. J., Pastink, A., and Heyting, C. (2006) Two levels of interference in mouse meiotic recombination. Proc. Natl. Acad. Sci. USA 103, 9607–9612.
Muller, H. J. (1916) The mechanism of crossing-over. IV. The manner of occurrence of crossing-over. The American Naturalist 50, 284–385.
Broman, K. W., Rowe, L. B., Churchill, G. A., and Paigen, K. (2002) Crossover interference in the mouse. Genetics 160, 1123–1131.
McPeek, M. S. and Speed, T. P. (1995) Modeling interference in genetic recombination. Genetics 139, 1031–1044.
de Boer, E. and Heyting, C. (2006) The diverse roles of transverse filaments of synaptonemal complexes in meiosis. Chromosoma 115, 220–234.
Zickler, D. and Kleckner, N. (1999) Meiotic chromosomes: integrating structure and function. Annu. Rev. Genet. 33, 603–754.
Sherman, J. D. and Stack, S. M. (1995) Two-dimensional spreads of synaptonemal complexes from solanaceous plants. IV. High-resolution recombination nodule map for tomato (Lycopersicon esculentum). Genetics 141, 683–708.
Anderson, L. K. and Stack, S. M. (2005) Recombination nodules in plants. Cytogenet. Genome Res. 109, 198–204.
Marcon, E. and Moens, P. (2003) MLH1p and MLH3p localize to precociously induced chiasmata of okadaic-acid-treated mouse spermatocytes. Genetics 165, 2283–2287.
Wang, H. and Höög, C. (2007) Structural damage to meiotic chromosomes impairs DNA recombination and checkpoint control in mammalian oocytes. J. Cell Biol. 173, 485–495.
Argueso, J. L., Wanat, J., Gemici, Z., and Alani, E. (2004) Competing crossover pathways act during meiosis in Saccharomyces cerevisiae. Genetics 168, 1805–1816.
de los Santos, T., Hunter, N., Lee, C., Larkin, B., Loidl, J., and Hollingsworth, N. M. (2003) The Mus81/Mms4 endonuclease acts independently of double-Holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast. Genetics 164, 81–94.
Lhuissier, F. G. P., Offenberg, H. H., Wittich, P. E., Vischer, N. O. E., and Heyting, C. (2007) The mismatch repair protein MLH1 marks a subset of strongly interfering crossovers in tomato. The Plant Cell 19, 862–876.
Franklin, F. C., Higgins, J. D., Sanchez-Moran, E., Armstrong, S. J., Osman, K. E., Jackson, N., et al. (2006) Control of meiotic recombination in Arabidopsis: role of the MutL and MutS homologues. Biochem. Soc. Trans. 34, 542–544.
Lawrie, N. M., Tease, C., and Hultén, M. A. (1995) Chiasma frequency, distribution and interference maps of mouse autosomes. Chromosoma 104, 308–314.
Moens, P. B., Heyting, C., Dietrich, A. J. J., van Raamsdonk, W., and Chen, Q. (1987) Synaptonemal complex antigen location and conservation. J. Cell Biol. 105, 93–103.
Froenicke, L., Anderson, L. K., Wienberg, J., and Ashley, T. (2002) Male mouse recombination maps for each autosome identified by chromosome painting. Am. J. Hum. Genet. 71, 1353–1368.
Baker, S. M., Plug, A. W., Prolla, T. A., Bronner, C. E., Harris, A. C., Yao, X., et al. (1996) Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat. Genet. 13, 336–342.
Lhuissier, F. G. P., Offenberg, H. H., Wittich, P. E., Vischer, N. O. E., and Heyting, C. (2007) The mismatch repair protein MLH1 marks a subset of strongly interfering crossovers in tomato. The Plant Cell 19, 862–876.
Colombo, P. C. and Jones, G. H. (1997) Chiasma interference is blind to the centromere. Heredity 79, 214–227.
Stam, P. (1979) Interference in genetic crossing over and chromosome mapping. Genetics 92, 573–594.
Jones, G. H. and Franklin, F. C. (2006) Meiotic crossing-over: obligation and interference. Cell 126, 246–248.
Falque, M., Mercier, R., Mezard, C., de Vienne, D., and Martin, O. C. (2007) Patterns of recombination and MLH1 foci density along mouse chromosomes: modeling effects of interference and obligate chiasma. Genetics 176, 1453–1467.
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de Boer, E., Lhuissier, F.G., Heyting, C. (2009). Cytological Analysis of Interference in Mouse Meiosis. In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 558. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-103-5_21
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DOI: https://doi.org/10.1007/978-1-60761-103-5_21
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