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Mouse Models of MMP and TIMP Function

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Matrix Metalloproteinase Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 622))

Abstract

As their name implies, matrix metalloproteinases (MMPs) are thought to be responsible for the turnover of connective tissue proteins, a function that is indeed performed by some family members. However, matrix degradation is possibly not the predominant function of these enzymes. Several studies have demonstrated that MMPs also act on a variety of non-matrix extracellular proteins, such as cytokines, chemokines, receptors, junctional proteins, and antimicrobial peptides, to mediate a wide range of biological processes, such as repair, immunity, and angiogenesis. Our understanding of the many, diverse and, at times, unexpected functions of MMPs largely arose from the use of gene-targeted mice. In this chapter, we discuss the phenotypes of some MMP-deficient and TIMP-null mice and strategies and pitfalls in targeted mutagenesis.

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Authors and Affiliations

  1. Center for Lung Biology, University of Washington School of Medicine, Seattle, WA, USA

    Sean E. Gill, Sean Y. Kassim, Timothy P. Birkland & William C. Parks

Authors
  1. Sean E. Gill
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  2. Sean Y. Kassim
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  3. Timothy P. Birkland
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  4. William C. Parks
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Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of East Anglia, Norwich, NR47TJ, United Kingdom

    Ian M. Clark

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Gill, S.E., Kassim, S.Y., Birkland, T.P., Parks, W.C. (2010). Mouse Models of MMP and TIMP Function. In: Clark, I. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology, vol 622. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-299-5_2

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  • DOI: https://doi.org/10.1007/978-1-60327-299-5_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-298-8

  • Online ISBN: 978-1-60327-299-5

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