Abstract
Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a relatively new mass-analysis method suitable for the analysis of biological molecules (1–3). Compared to other mass-analysis methods, MALDI-MS is based on simpler instrumentation that is more easily operated and so is more readily accessible to laboratories without prior experience in mass spectrometry. In addition, the method is sensitive and capable of high throughput. Its use in biochemical laboratories is already widespread, despite its recent introduction and the continued development of MALDI-MS instrumentation and applications.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hillenkamp, F., Karas, M., Beavis, R., and Chait, B. (1991) Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. Anal. Chem. 63, 1193–1202.
Cotter, R. J. (1992) Time-of-flight mass spectrometry for the structural analysis of biological molecules. Anal. Chem. 64, 1027–1039.
Chait, B. T. and Kent, S. H. (1992) Weighing naked proteins: practical, high-accuracy mass measurements of peptides and proteins. Science 257, 1885–1894.
Brown, J. L. and Roberts, W. K. (1976) Evidence that approximately eighty percent of the soluble proteins from Ehrlich Asites cells are amino terminally acetylated. J. Biol. Chem. 251, 1009–1014.
Driessen, H. P., De Jong, W. W., Tesser, G. I., and Bloemendal, H. (1985) The mechanism of N-termimal acetylation of proteins, in Critical Reviews in Biochemistry, vol. 8 (Fasman, G. D., ed.), CRC, Boca Raton, FL, pp. 281–325.
Rosenfeld, J., Capdeville, J., Guillemot, J., and Ferrara, P. (1992) In-gel digestions of proteins for internal sequence analysis after 1 or 2 dimensional gel electrophoresis. Anal. Biochem. 203, 173–179.
Williams, K. R. and Stone, K. S. (1995) In-gel digestion of SDS PAGE-separated proteins: observations from internal sequencing of 25 proteins, in Techniques in Protein Chemistry VI (Crabb, J., ed.), Academic, San Diego, pp. 143–152.
Stone, K. L., McNulty, D. E., LoPresti, M. L., Crawford, J. M., DeAngelis, R., and Williams, K. R. (1992) Elution and internal amino acid sequencing of PVDF-blotted proteins, in Techniques in Protein Chemistry III (Angeletti, R., ed.), Academic, New York, pp. 22–34.
Williams, K. R., Kobayashi, R., Lane, W., and Tempst, P. (1993) Internal amino acid sequencing: observations from four different laboratories. ABRF News 4, 7–12.
Stone, K. L. and Williams, K. R. (1993) Enzymatic digestion of proteins and HPLC peptide isolation, in A Practical Guide to Protein and Peptide Purification for Microsequencing (Matsudaira, P. T., ed.), Academic, New York, pp. 43–69.
Elliott, J. I., Stone, K. L., and Williams, K. R. (1993) Synthesis and use of an internal amino acid sequencing standard peptide. Anal. Biochem. 211, 94–101.
Henzel, W. J., Billed, T. M., Stults, J. T., Wong, S. C., Grimley, C., and Watanabe, C. (1993) Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc. Natl. Acad. Sci. USA 90, 5011–5015.
Williams, K. R. and Carr, S. A. (1995) Protein analysis by integrated sample preparation, chemistry, and mass spectrometry, in Encyclopedia of Molecular Biology and Biotechnology (Meyers, R. A., ed.), VCH Publishers, New York, pp. 731–737.
Bornsen, K. O., Schar, M., Gassman, E., and Steiner, V. (1991) Analytical applications of matrix-assisted laser desorption ionization mass spectrometry. Biol. Mass. Spectrom. 20, 471–478.
Germanos, S., Casteels, P., Elicone, C., Powell, M., and Tempst, P. (1994) Combined Edman-chemical and laser-desorption mass spectrometric approaches to micro peptide sequencing: optimization and applications, in Techniques in Protein Chemistry V (Crabb, J. W., ed.), Academic, New York, pp. 143–150.
Mann, M., Horup, P., and Roepstorff, P. (1993) Use of mass spectrometric molecular weight information to identify proteins in sequence databases. Biol. Mass. Spectrom. 22, 338–345.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Williams, K.R., Samandar, S.M., Stone, K.L., Saylor, M., Rush, J. (1996). Matrix-Assisted Laser Desorption Ionization Mass Spectrometry as a Complement to Internal Protein Sequencing. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_91
Download citation
DOI: https://doi.org/10.1007/978-1-60327-259-9_91
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
eBook Packages: Springer Book Archive