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Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

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The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The comparison of the primary structure of proteins is an important facet in the characterization of families of proteins from the same organism, similar proteins from different organisms, and cloned gene products. There are many methods available to establish the sequence similarities of proteins. A relatively uncomplicated approach is to compare the peptide fragments of proteins generated by enzymatic or chemical cleavage, i.e., peptide mapping. The similarity or dissimilarity of the resultant peptides reflects the similarity or dissimilarity of the parent proteins.

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References

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Author information

Authors and Affiliations

  1. Division of Biological Sciences, University of Montana, Missoula, MT

    Ralph C. Judd

Authors
  1. Ralph C. Judd
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 1996 Humana Press Inc., Totowa, NJ

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Judd, R.C. (1996). Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_74

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  • DOI: https://doi.org/10.1007/978-1-60327-259-9_74

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

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