Abstract
O-linked oligosaccharides having the core sequences shown below can be released specifically from protein via a β-elimination reaction catalyzed by alkali. The reaction is usually carried out with concomitant reduction to prevent peeling, a reaction caused by further β-elimination around the ring of 3-substituted monosaccharides (1). The reduced oligosaccharides can be specifically bound by solid sorbent extraction on phenylboronic acid (PBA) columns (2).
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References
Hounsell, E. F. (1994) Physicochemical analyses of oligosaccharides determinants of glycoproteins. Adv. Carbohyd. Chem. Biochem. 30, 311–350.
Stoll, M. S. and Hounsell, E. F. (1988) Selective purification of reduced oligosaccharides using a phenylboronic acid bond elut column: potential application in HPLC, mass spectrometry, reductive amination procedures and antigenic/serum analysis. Biomed. Chromatogr. 2, 24–253.
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© 1996 Humana Press Inc., Totowa, NJ
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Hounsell, E.F., Davies, M.J., Smith, K.D. (1996). Chemical Release of O-Linked Oligosaccharide Chains. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_105
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DOI: https://doi.org/10.1007/978-1-60327-259-9_105
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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