Summary
Bacteria communicate with other members of their community through the secretion and perception of small chemical cues or signals. The recognition of a signal normally leads to the expression of a large suite of genes, which in some bacteria are involved in the regulation of virulence factors, and as a result, these signaling compounds are key regulatory factors in many disease processes. Thus, it is of interest when studying pathogens to understand the mechanisms used to control the expression of virulence genes so that strategies might be devised for the control of those pathogens. Clearly, the ability to interfere with this process of signaling represents a novel approach for the treatment of bacterial infections. There is a broad range of compounds that bacteria can use for signaling purposes, including fatty acids, peptides, N-acylated homoserine lactones, and the signals collectively called autoinducer 2 (AI-2). This chapter will focus on the latter two signaling systems as they are present in a range of medically relevant bacteria, and here we describe assays for determining whether an organism produces a particular signal and assays that can be used to identify inhibitors of the signaling cascade. Lastly, the signal detection and inhibition assays will be directly linked to the expression of virulence factors of specific pathogens.
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References
Fuqua, C. and Greenberg, E. P. (2002) Listening in on bacteria: acyl-homoserine lactone signalling. Nat. Rev. Mol. Cell Biol. 3, 685–695.
Venturi, V. (2006) Regulation of quorum sensing in Pseudomonas. FEMS Microbiol. Rev. 30, 274–291.
Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W., and Greenberg, E. P. (1998) The involvement of cell-cell signals in the development of a bacterial biofilm. Science 280, 295–298.
Wu, H., Song, Z., Hentzer, M., Andersen, J. B., Molin, S., Givskov, M., and Hoiby, N. (2004) Synthetic furanones inhibit quorum-sensing and enhance bacterial clearance in Pseudomonas aeruginosa lung infection in mice. J. Antimicrob. Chemother. 53, 1054–1061.
Zhu, H., Bandara, R., Conibear, T., Thuruthyil, S. J., Rice, S. A., Kjelleberg, S., Givskov, M., and Willcox, M. D. P. (2004) Pseudomonas aeruginosa with lasI quorum-sensing deficiency is avirulent during corneal infection. Invest. Ophthalmol. Vis. Sci. 45, 1897–1903.
Huber, B., Riedel, K., Hentzer, M., Heydorn, A., Gotschlich, A., Givskov, M., Molin, S., and Eberl, L. (2001) The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility. Microbiology 147, 2517–2528.
Labbate, M., Queck, S. Y., Koh, K. S., Rice, S. A., Givskov, M., and Kjelleberg, S. (2004) Quorum sensing controlled biofilm development in Serratia liquefaciens MG1. J. Bacteriol. 186, 692–698.
Riedel, K., Ohnesorg, T., Krogfelt, K. A., Hansen, T. S., Omori, K., Givskov, M., and Eberl, L. (2001) N-Acyl-L-homoserine lactone-mediated regulation of the Lip secretion system in Serratia liquefaciens MG1. J. Bacteriol. 183, 1805–1809.
McDougald, D., Rice, S. A., and Kjelleberg, S. (2001) SmcR-dependent regulation of adaptive phenotypes in Vibrio vulnificus. J. Bacteriol. 183, 758–762.
Sperandio, V., Torres, A. G., Giron, J. A., and Kaper, J. B. (2001) Quorum sensing is a global regulatory mechanism in enterohemorrhagic Escherichia coli O157:H7. J. Bacteriol. 183, 5187–5197.
Federle, M. J. and Bassler, B. L. (2003) Interspecies communication in bacteria. J. Clin. Invest. 112, 1291–1299.
Linkous, D. A. and Oliver, J. D. (1999) Pathogenesis of Vibrio vulnificus. FEMS Microbiol. Lett. 174, 207–214.
Campos, L. C., Zahner, V., Avelar, K. E. S., Alves, R. M., Pereira, D. S. G., Vital Brazil, J. M., Freitas, F. S., Salles, C. A., and Karaolis, D. K. R. (2004) Genetic diversity and antibiotic resistance of clinical and environmental Vibrio cholerae suggests that many serogroups are reservoirs of resistance. Epidemiol. Infect. 132, 985–992.
Schmidt, F. R. (2004) The challenge of multidrug resistance: actual strategies in the development of novel antibacterials. Appl. Microbiol. Biotechnol. 63, 335–343.
Clark, D. J. and Maaloe, O. (1967) DNA replication and the division cycle in Escherichia coli. J. Mol. Biol. 23, 99–112.
Greenberg, E. P., Hastings, J. W., and Ulitzer, S. (1979) Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria. Arch. Microbiol. 120, 87–91.
Bertani, G. (1951) Studies on lysogenesis. J. Bacteriol. 62, 293–300.
Andersen, J. B., Heydorn, A., Hentzer, M., Eberl, L., Geisenberger, O., Christensen, B. B., Molin, S., and Givskov, M. (2001) gfp-based N-acyl homoserine lactone sensor systems for detection of bacterial communication. Appl. Environ. Microbiol. 67, 575–585.
Rice, S. A., Kjelleberg, S., Givskov, M., De Boer, W., and Chernin, L. (2004) In situ detection of bacterial quorum sensing signal molecules, in Molecular Microbial Ecology Manual (Kowalchuck, G. A., Ed.), Dordrecht/Boston/London, pp. 1629–1650, Kluwer Academic Publishers.
Beatson, S. A., Whitchurch, C. B., Semmler, A. B. T., and Mattick, J. S. (2002) Quorum sensing is not required for twitching motility in Pseudomonas aeruginosa. J. Bacteriol. 184, 3598–3604.
Pesci, E. C., Pearson, J. P., Seed, P. C., and Iglewski, B. H. (1997) Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 179, 3127–3132.
Pearson, J. P., Pesci, E. C., and Iglewski, B. (1997) Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in contro l of elastase and rhamnolipid biosynthesis genes. J. Bacteriol. 179, 5756–5767.
Bassler, B. and Silverman, M. R. (Eds.) (1995) Two component Signal Transduction. Intercellular communication in marine Vibrio species: density-dependent regulation of the expression of bioluminescence, ASM Press, Washington, DC, pp. 431–444.
Holden, M. T. G., Chahabra, S. R., deNys, R., Stead, P., Bainton, N. J., Hill, P. J., Manefield, M., Kumar, N., Labbate, M., England, D., Rice, S. A., Givskov, M., Salmond, G., Stewart, G. S. A. B., Bycroft, B. W., Kjelleberg, S., and Williams, P. (1999) Quorum sensing cross talk: isolation and chemical characterisation of cyclic dipeptides from Pseudomonas aeruginosa and other Gram-negative bacteria. Mol. Microbiol. 33, 1254–1266.
Nealson, K. H., Eberhard, A., and Hastings, J. W. (1972) Catabolite repression of bacterial bioluminescence: functional implications. Proc. Natl. Acad. Sci. USA 69, 1073–1076.
Turovskiy, Y. and Chikindas, M. L. (2006) Autoinducer-2 bioassay is a qualitative, not quantitative method influenced by glucose. J. Microbiol. Methods 66, 497–503.
Acknowledgments
This work has been supported by the National Health and Medical Research Council of Australia and funding from Biosignal Ltd, Australia.
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Rice, S.A., McDougald, D., Givskov, M., Kjelleberg, S. (2008). Detection and Inhibition of Bacterial Cell–Cell Communication. In: DeLeo, F.R., Otto, M. (eds) Bacterial Pathogenesis. Methods in Molecular Biology™, vol 431. Humana Press. https://doi.org/10.1007/978-1-60327-032-8_5
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DOI: https://doi.org/10.1007/978-1-60327-032-8_5
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